Bilirubin helps to overcome the two-cell block in mouse oocyte cultures.

Bilirubin helps to overcome the two-cell block in mouse oocyte cultures. Research Abstract Details 

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Bilirubin helps to overcome the two-cell block in mouse oocyte cultures. Abstract Text:

PURPOSE: In vitro fertilization and culture of mouse oocytes, under normal atmospheric oxygen tension, subjects them to severe oxidative stress. Oocytes from some strains of mice lack the natural protective mechanism that guards them against this oxidative stress and fail to develop beyond the two-cell stage. METHODS: We could overcome the toxic effects of oxygen metabolites by adding 0.2-0.4 mg/dl bilirubin in a lactate-pyruvate culture medium defined by Whitten (1971). Six- to 8-week-old ICR (Institute of Cancer Research) female mice were super ovulated by intra peritoneal injection of 5 IU PMSG (pregnant mare serum gonadotropin) followed by 10 IU hCG 48 h later. The oocytes were collected from the distended fallopian tubes and inseminated with 1-2 million sperm from 3-4-month-old ICR male mice. The eggs were scored at 24, 48, and 72 h after the hCG injection. CONCLUSIONS: With 0.4 mg/dl bilirubin supplement, by the end of 72 h, 82% of the eggs progressed from the two-cell stage to the four-cell stage. Routine inclusion of bilirubin can improve embryo development in vitro.

Bilirubin helps to overcome the two-cell block in mouse oocyte cultures. Publishing Authors By Initials

For similar inorganic chemicals: free radicals: reactive oxygen species research abstracts see: inorganic chemicals: free radicals: reactive oxygen species research

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Bilirubin helps to overcome the two-cell block in mouse oocyte cultures. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 11

Page Numbers: 510-4

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Nov

YEAR: 1994

Bilirubin helps to overcome the two-cell block in mouse oocyte cultures. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Bilirubin helps to overcome the two-cell block in mouse oocyte cultures. Keywords Mesh Terms:

KEYWORDS: Reactive Oxygen Species

MESH TERMS: embryology

Chemical & Substance for Abstract: Bilirubin helps to overcome the two-cell block in mouse oocyte cultures. Information

Substance Name: Bilirubin

Registry Number: 635-65-4

Grant and Affiliation Information for Bilirubin helps to overcome the two-cell block in mouse oocyte cultures.

AFFILIATION: Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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