Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase.

Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. Research Abstract Details 

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Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. Abstract Text:

Izumi Orita,Naoki Sakamoto,Nobuo Kato,Hiroya Yurimoto,Yasuyoshi Sakai,Izumi Orita,Naoki Sakamoto,Nobuo Kato,Hiroya Yurimoto,Yasuyoshi Sakai,

The formaldehyde-fixing enzymes, 3-Hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexuloisomerase (PHI), are the key enzymes catalyzing sequential reactions in the ribulose monophosphate (RuMP) pathway. In this study, we generated two fused gene constructs of the hps and phi genes (i.e., hps-phi and phi-hps) from a methylotrophic bacterium Mycobacterium gastri MB19. The gene product of hps-phi exhibited both HPS and PHI activities at room temperature and catalyzed the sequential reactions more efficiently than a simple mixture of the individual enzymes. The gene product of phi-hps failed to display any enzyme activity. Escherichia coli strains harboring the hps-phi gene consumed formaldehyde more efficiently and exhibited better growth in a formaldehyde-containing medium than the host strain. Our results demonstrate that the engineered fusion gene has the possibility to be used to establish a formaldehyde-resistance detoxification system in various organisms.

Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. Publishing Authors By Initials

I Orita,N Sakamoto,N Kato,H Yurimoto,Y Sakai,I Orita,N Sakamoto,N Kato,H Yurimoto,Y Sakai,

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Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied microbiology and biotechnology

VOLUME: 76

Page Numbers: 439-45

Journal Abbreviation: Appl. Microbiol. Biotechnol.

ISSN: 0175-7598

DAY: 23

MONTH: 05

YEAR: 2007

Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. Information

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LANGUAGE: eng

NlmUniqueID: 8406612

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Grant and Affiliation Information for Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase.

AFFILIATION: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, 606-8502 Kyoto, Japan.

Country: Germany

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MEDLINETA: Appl Microbiol Biotechnol

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