beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha.

beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha. Research Abstract Details 

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beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha. Abstract Text:

T Yano,Y Hayashi,S Yamamoto,

A sensitive and specific enzyme immunoassay for prostaglandin F2 alpha was developed in which prostaglandin F2 alpha was labeled with beta-D-galactosidase. After competitive binding to antibody between enzyme-labeled and free prostaglandin F2 alpha, the immunoreactive product was precipitated by double antibody technique, and the enzyme activity of the precipitate was determined fluorometrically. The procedures allowed determination of 0.03-20 pmol of prostaglandin F2 alpha. The detection limit of 0.03 pmol was comparable with that of previously reported radioimmunoassays. Enzyme immunoassay and radioimmunoassay showed almost the same cross-reactivity with other prostaglandins and metabolites.

beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha. Publishing Authors By Initials

T Yano,Y Hayashi,S Yamamoto,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research

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beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 90

Page Numbers: 773-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1981

beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha. Information

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LANGUAGE: eng

NlmUniqueID: 376600

beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: analysis

Chemical & Substance for Abstract: beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha. Information

Substance Name: beta-Galactosidase

Registry Number: EC 3.2.1.23

Grant and Affiliation Information for beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha.

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Country: JAPAN

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MEDLINETA: J Biochem

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