beta-Cell Specific Cytoprotection by Prolactin on Human Islets.

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beta-Cell Specific Cytoprotection by Prolactin on Human Islets. Abstract Text:

T Yamamoto,C Ricordi,A Mita,A Miki,Y Sakuma,R D Molano,A Fornoni,A Pileggi,L Inverardi,H Ichii,

INTRODUCTION: Many cytoprotective agents have been reported to improve islet isolation and transplantation outcomes. However, several of these agents improve all cell subsets within an islet preparation; selection of non-beta-cell components (eg, acinar cells) may have a negative effect on beta-cell function and survival. In this study, we examined the effect of prolactin (PRL) supplementation in the culture medium to determine whether it exerted beta-cell-selective cytoprotection on islet viability and function. MATERIALS AND METHODS: Human islets were precultured with or without recombinant human PRL (500 mug/L) for 48 hours. The fractional viability and cellular composition of non-beta-cell and beta-cell-specific components were assessed using FACS and Laser Scanning Cytometry (LSC). Islet potency was assessed in vivo by transplantation into chemically induced diabetic immunodeficient mice. RESULTS: The relative viable beta-cell mass and the relative islet beta-cell content in the PRL group were 28% higher (P = .018) and 19% higher (P = .029) than the control group, respectively. All transplanted mice achieved normoglycemia in both groups, indicating that PRL treatment did not alter islet function. CONCLUSION: PRL treatment improved beta-cell-specific viability and survival of human islets in vitro. The development of novel beta-cell-specific cytoprotective strategies may be of assistance in improving islet transplantation.

beta-Cell Specific Cytoprotection by Prolactin on Human Islets. Publishing Authors By Initials

T Yamamoto,C Ricordi,A Mita,A Miki,Y Sakuma,RD Molano,A Fornoni,A Pileggi,L Inverardi,H Ichii,

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beta-Cell Specific Cytoprotection by Prolactin on Human Islets. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Transplantation proceedings

VOLUME: 40

Page Numbers: 382-3

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ISSN: 0041-1345

DAY: 31

MONTH: Mar

YEAR: 2008

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LANGUAGE: eng

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Country: United States

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MEDLINETA: Transplant Proc

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