Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant.

Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant. Abstract Text:

Xiao-Guang Luo,Hong Wang,Jin Zhou,Rong Yan,Zhe Wu,Chao-Dong Zhang,Qiu-Shuang Wang,

Objective The microglias is the representative of immune cells in the brain. It plays dual roles of both repairing and damaging in injured nervous system, and works as an inevitable component of the circumstance of injured neurons. This study was aiming at the effects of the microglias on the biological activities of mesenchymal stem cells (MSCs) in the circumstance of injured neurons. Methods MSCs were obtained by primary culture. We adopted PC12 cells (PC12) and BV2 cells (BV2) to substitute for neurons and microglias, respectively. PC12 were injured by aged Abeta(1-40) and the supernatant of the injured PC12 was used to set up the circumstance of injured neurons. Transwells were used for co-culture of BV2 and MSCs, which allowed the independent detection of cells after co-culture. Immunofluorescence was used to identify MSCs and neuron-differentiating cells with CD44 and neuron specific enolase (NSE) staining, respectively. MTT assay was adopted to measure the proliferation. Results In the circumstance of both BV2 presence and injured PC12 supernatant incubation, either the proliferation or the differentiation of MSCs reached the highest, which seemed to be contradictory, but we gave our explanations. With the BV2 co-culture, the proliferation of MSCs tend to be higher, but the neuron-differentiating MSCs were similar to those incubated without BV2 co-culture either in normal or injured in PC12 supernatant. With the incubation of injured PC12 supernatant, the neuron-differentiating cells were significantly higher than that of control (P < 0.05). Conclusion In the circumstance of injured neurons, microlgias tend to promote the MSCs proliferation. Although not helpful in neuron-differentiating, microglias did not exert any negative effect either.

Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant. Publishing Authors By Initials

XG Luo,H Wang,J Zhou,R Yan,Z Wu,CD Zhang,QS Wang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Neuroscience bulletin

VOLUME: 22

Page Numbers: 221-6

Journal Abbreviation:

ISSN: 1673-7067

DAY: 20

MONTH: Jul

YEAR: 2006

Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101256850

Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant.

AFFILIATION: Department of Neurology, First affiliated Hospital, China Medical University, Shenyang 110001, China; E-mail: chunlinge@yahoo.com.cn.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Neurosci Bull

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Beneficial effects of BV2 cell on proliferation and neuron-differentiating of mesenchymal stem cells in the circumstance of injured PC12 cell supernatant Related Publications

• A novel link between the proteasome pathway and the signal transduction pathway of the bone morphogenetic proteins (BMPs).
• Effects of phytoestrogen genistein on delayed after depolarization and triggered activity induced by ouabain in guinea pig papillary muscles.
• Occupational health in China.
• Research on expression of telomerase in human odontogenic lesions.
• Suppression of tumor necrosis factor-mediated apoptosis by nuclear factor kappaB-independent bone morphogenetic protein/Smad signaling.
• [A gene analysis of the low density lipoprotein receptor in Chinese with homozygous familial hyperchol-esterolemia]
• [A novel rat aplastic anemia model induced by 5-fluorouracil combined with busulfan]
• [A study of association between atherothrombotic brain infarction and HLA-DQA1 peptide binding motifs]
• [An experimental study on the bone repairing ability of recombinant human bone morphogenetic protein-2-coral composited artificial bone]
• [Analysis of EEG based on the complexity measure]
• [Apoptosis of bone marrow cells aplastic anemia patients treated with cyclosporin A]
• [Carrier detection and prenatal diagnosis for hemophilia A]
• [Changes of the expression of integrin alpha6beta1 and the activity of focal adhesion kinase in the liver sinusoidal endothelial cell in experimental fibrotic rats]
• [Clinical analysis of 22 patients with primary biliary cirrhosis]
• [Clinical significance of hemostatic molecular markers in acute leukemia]
• [Effects of phenytoin on structural aberration of human sperm chromosomes in vitro]
• [Expression and purification of human papillomavirus type16 L1 protein in a prokaryotic expression system]
• [Extension of cardiac monitoring function by used of ordinary ECG machine]
• [Histopathological features of 27 cases of primary biliary cirrhosis]
• [Multiagent system for individual artificial hip joint]
• [Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells]
• [No evidence for association between the alpha 2-macroglobulin polymorphism and Alzheimer's disease in the Han Chinese]
• [PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene]
• [Preliminary study of prevention from restenosis of hepatic venous in stents by gene transfer of phVEGF165]
• [Prostaglandin inhibitor indomethacin inhibits afferent activities of Adelta and C units in the saphenous nerve of diabetic hyperalgesic rats]
• [Research and development on microwave sterilization]
• [Role of recombinant human bone morphogenetic protein 2/collagen as an onlay bone graft on adult rat calvarial bone]
• [Study of telomerase activity and its related genes expression in leukemia cells]
• [Thrombopoietic effect of recombinant human thrombopoietin gene transferred to mice mediated by electric pulse on normal and experimental thrombocytopenia mice]
• [Thymectomy for late-onset myasthenia gravis in the elderly (with report of 23 cases)]