Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip.

Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip. Research Abstract Details 

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Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip. Abstract Text:

Amanda J Haes,Alex Terray,Greg E Collins,

A microchip-based, displacement immunoassay for the sensitive laser-induced fluorescence detection of staphylococcal enterotoxin B is presented. The glass microchip device consists of a microchannel that contains a double weir structure for supporting antibody-functionalized microbeads. After a 30-min sample preparation step, the displacement assay was performed without user intervention and produced quantitative results in an additional 20 min. Linear detection responses were observed over 6 orders of magnitude and provided detection limits down to 1 fM (28.5 fg/mL). The surprisingly low detection limits are hypothesized to arise from field-based enrichment analogous to field-amplified stacking, chromatographic effects, and limited diffusion lengths in the microbead bed. The assay was challenged with bovine serum albumin, casein, and milk sample matrixes. This system has the potential to provide highly sensitive detection capabilities for target biomolecules.

Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip. Publishing Authors By Initials

AJ Haes,A Terray,GE Collins,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 78

Page Numbers: 8412-20

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 15

MONTH: Dec

YEAR: 2006

Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip. Information

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LANGUAGE: eng

NlmUniqueID: 370536

Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: analysis

Chemical & Substance for Abstract: Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip. Information

Substance Name: enterotoxin B, staphylococcal

Registry Number: 39424-53-8

Grant and Affiliation Information for Bead-assisted displacement immunoassay for staphylococcal enterotoxin B on a microchip.

AFFILIATION: Chemistry Division, Naval Research Laboratory, 4555 Overlook Avenue, SW, Code 6112, Washington, DC 20375-5342, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI056047

ACRONYM: AI

MEDLINETA: Anal Chem

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