Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides.

Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Research Abstract Details 

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Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Abstract Text:

J A Gomez,V Gama,T Yoshida,W Sun,P Hayes,K Leskov,D Boothman,S Matsuyama,

We found that Ku70, a known DNA repair factor, has a novel function to bind and inhibit Bax (Bcl-2-associated X protein), a key mediator of apoptosis. Pentapeptides derived from the Bax-binding domain of Ku70 were cell-permeable and protected cells from Bax-mediated apoptosis. These pentapeptides were called BIPs (Bax-inhibiting peptides). BIPs may become a useful therapeutic tool to reduce cellular damage. We also generated BIP mutant pentapeptides that do not inhibit Bax, but retain their cell-penetrating activity. Since both BIPs and BIP mutants are cell-permeable, these peptides were designated CPP5s (cell-penetrating pentapeptides). Among the CPP5s discovered, VPTLK (BIP) and KLPVM (BIP mutant) were confirmed to possess protein transduction activity by examination of the delivery of GFP (green fluorescent protein) into cells by these peptides. The mechanism of cell penetration by CPP5s is not known. CPP5s enter the cell at 0 and 4 degrees C. In preliminary studies, various inhibitors of endocytosis and pinocytosis did not show any significant suppression of CPP5 cell entry. CPP5s have very low toxicity in vitro and in vivo and so may be useful tools in order to develop non-toxic drug-delivery technologies.

Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Publishing Authors By Initials

JA Gomez,V Gama,T Yoshida,W Sun,P Hayes,K Leskov,D Boothman,S Matsuyama,

For similar peptides: intracellular signaling peptides and proteins: apoptosis regulatory proteins: proto-oncogene proteins c-bcl-2: bcl-2-associated x protein research abstracts see: peptides: intracellular signaling peptides and proteins: apoptosis regulatory proteins: proto-oncogene proteins c-bcl-2: bcl-2-associated x protein research

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Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Journal Published:

PUBLICATION TYPE: Review

Journal: Biochemical Society transactions

VOLUME: 35

Page Numbers: 797-801

Journal Abbreviation: Biochem. Soc. Trans.

ISSN: 0300-5127

DAY: 3

MONTH: Aug

YEAR: 2007

Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Information

Number of References: 39

LANGUAGE: eng

NlmUniqueID: 7506897

Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Keywords Mesh Terms:

KEYWORDS: bcl-2-Associated X Protein

MESH TERMS: antagonists & inhibitors

Chemical & Substance for Abstract: Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Information

Substance Name: bcl-2-Associated X Protein

Registry Number: 0

Grant and Affiliation Information for Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides.

AFFILIATION: Pharmacology Department, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.

Country: England

AGENCY: United States NCI

GRANT: P20CA10373

ACRONYM: CA

MEDLINETA: Biochem Soc Trans

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