Basement membrane proteins play an important role in the invasive processes of human pancreatic cancer cells.

Basement membrane proteins play an important role in the invasive processes of human pancreatic cancer cells. Research Abstract Details 

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Basement membrane proteins play an important role in the invasive processes of human pancreatic cancer cells. Abstract Text:

Hirozumi Sawai,Yuji Okada,Hitoshi Funahashi,Hiroki Takahashi,Yoichi Matsuo,Akira Yasuda,Nobuo Ochi,Hiromitsu Takeyama,Tadao Manabe,Hirozumi Sawai,Yuji Okada,Hitoshi Funahashi,Hiroki Takahashi,Yoichi Matsuo,Akira Yasuda,Nobuo Ochi,Hiromitsu Takeyama,Tadao Manabe,Hirozumi Sawai,Yuji Okada,Hitoshi Funahashi,Hiroki Takahashi,Yoichi Matsuo,Akira Yasuda,Nobuo Ochi,Hiromitsu Takeyama,Tadao Manabe,

BACKGROUND: The invasive interaction between cells and their matrix has important roles in tumor cell invasion. This study investigated modulation of basement membrane (BM) proteins, especially collagen IV (Coll IV), laminin, and fibronectin (FN), in invasion of human pancreatic cancer cells. Furthermore, we examined the roles of beta(1)-integrins and arginine-glycine-aspartic (RGD)-containing oligopeptide in cell-matrix interactions. MATERIALS AND METHODS: Expression of integrins were examined by reverse transcriptase-polymerase chain reaction and flow-cytometric analysis in three human pancreatic cancer cell lines (BxPC-3, PANC-1, and SW1990), respectively. To determine the effect of BM proteins, invasion assays were performed. Western blot analysis for extracellular signal-regulated kinase (ERK) was performed to investigate the involvement of ERK1/2 signaling pathways. RESULTS: BM proteins significantly enhanced the invasive behavior of pancreatic cancer cells. Pretreatment with anti-beta(1)-integrin antibody suppressed invasion into Matrigel, but RGD-containing peptide inhibited invasion, which was enhanced by Coll IV and FN, not laminin. Treatment with both RGD-containing peptide and beta(1)-integrin antibody inhibited ERK1/2 phosphorylation activated by Coll IV and FN. CONCLUSIONS: BM proteins have positive actions on the processes of pancreatic cancer cell invasion and cross-talk between BM proteins and beta(1)-integrins widely participates in the multistep processes of pancreatic cancer invasion and metastasis formation.

Basement membrane proteins play an important role in the invasive processes of human pancreatic cancer cells. Publishing Authors By Initials

H Sawai,Y Okada,H Funahashi,H Takahashi,Y Matsuo,A Yasuda,N Ochi,H Takeyama,T Manabe,H Sawai,Y Okada,H Funahashi,H Takahashi,Y Matsuo,A Yasuda,N Ochi,H Takeyama,T Manabe,H Sawai,Y Okada,H Funahashi,H Takahashi,Y Matsuo,A Yasuda,N Ochi,H Takeyama,T Manabe,

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Basement membrane proteins play an important role in the invasive processes of human pancreatic cancer cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of surgical research

VOLUME: 144

Page Numbers: 117-23

Journal Abbreviation: J. Surg. Res.

ISSN: 0022-4804

DAY: 3

MONTH: 08

YEAR: 2007

Basement membrane proteins play an important role in the invasive processes of human pancreatic cancer cells. Information

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LANGUAGE: eng

NlmUniqueID: 376340

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AFFILIATION: Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Country: United States

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MEDLINETA: J Surg Res

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