Bacterial surface display of GFP(uv) on bacillus subtilis spores.

Bacterial surface display of GFP(uv) on bacillus subtilis spores. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Bacterial surface display of GFP(uv) on bacillus subtilis spores. Abstract Text:

June-Hyung Kim,Changhyun Roh,Chang-Won Lee,Dohyun Kyung,Soo-Keun Choi,Heung-Chae Jung,Jae-Gu Pan,Byung-Gee Kim,June-Hyung Kim,Changhyun Roh,Chang-Won Lee,Dohyun Kyung,Soo-Keun Choi,Heung-Chae Jung,Jae-Gu Pan,Byung-Gee Kim,

To analyze a cotG-based Bacillus subtilis spore display system directly, GFP(uv) was expressed on the surface of Bacillus subtilis spores. When GFP(uv) was fused to the C-terminal of the cotG structural gene and expressed, the existence of a CotG-GFP(uv) fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This GFP(uv) displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.

Bacterial surface display of GFP(uv) on bacillus subtilis spores. Publishing Authors By Initials

JH Kim,C Roh,CW Lee,D Kyung,SK Choi,HC Jung,JG Pan,BG Kim,JH Kim,C Roh,CW Lee,D Kyung,SK Choi,HC Jung,JG Pan,BG Kim,

For similar cells: spores: spores, bacterial research abstracts see: cells: spores: spores, bacterial research

PUBMED ID PMID:

MEDLINE DATE:

Bacterial surface display of GFP(uv) on bacillus subtilis spores. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 677-80

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 3

MONTH: Apr

YEAR: 2007

Bacterial surface display of GFP(uv) on bacillus subtilis spores. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9431852

Bacterial surface display of GFP(uv) on bacillus subtilis spores. Keywords Mesh Terms:

KEYWORDS: Spores, Bacterial

MESH TERMS: genetics

Chemical & Substance for Abstract: Bacterial surface display of GFP(uv) on bacillus subtilis spores. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Bacterial surface display of GFP(uv) on bacillus subtilis spores.

AFFILIATION: Department of Chemical Engineering, Dong-A University, Busan 604-714, Korea.

Country: Korea (South)

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Microbiol Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Bacterial surface display of GFPuv on bacillus subtilis spores Related Publications

• Aberrant expression of MUC3 and MUC4 membrane-associated mucins and sialyl Le(x) antigen in pancreatic intraepithelial neoplasia.
• Aberrant expression of MUC5AC and MUC6 gastric mucins and sialyl Tn antigen in intraepithelial neoplasms of the pancreas.
• Advanced capabilities for future light sources.
• Aging of the population and the subsequent increase in the mistreatment of the elderly in Korea.
• Alkali-catalyzed beta-elimination of periodate-oxidized glycans: a novel method of chemical deglycosylation of mucin gene products in paraffin embedded sections.
• Altered mucin core peptide immunoreactivity in the colon polyp-carcinoma sequence.
• Basic fibroblast growth factor-induced translocation of p21-activated kinase to the membrane is independent of phospholipase C-gamma1 in the differentiation of PC12 cells.
• Biological properties and expression of mucins in 5-fluorouracil resistant HT29 human colon cancer cells.
• Cholangiocarcinomas arising in cirrhosis and combined hepatocellular-cholangiocellular carcinomas share apomucin profiles.
• Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans.
• Drynariae Rhizoma promotes osteoblast differentiation and mineralization in MC3T3-E1 cells through regulation of bone morphogenetic protein-2, alkaline phosphatase, type I collagen and collagenase-1.
• Elderly Women's Grief in Korea.
• Expression of MUC2, MUC5AC and MUC6 apomucins in carcinoma, dysplasia and non-dysplastic epithelia of the gallbladder.
• Goblet cell-specific expression mediated by the MUC2 mucin gene promoter in the intestine of transgenic mice.
• Identification and characterization of the MUC2 (human intestinal mucin) gene 5'-flanking region: promoter activity in cultured cells.
• Immunohistochemical study of mucin carbohydrates and core proteins in hepatolithiasis and cholangiocarcinoma.
• Induction of mucin gene expression in middle ear of rats by tumor necrosis factor-alpha: potential cause for mucoid otitis media.
• Initiation of transcription of the MUC3A human intestinal mucin from a TATA-less promoter and comparison with the MUC3B amino terminus.
• MUC3 human intestinal mucin. Analysis of gene structure, the carboxyl terminus, and a novel upstream repetitive region.
• Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells.
• Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor.
• Monoclonal antibodies against partially deglycosylated colon cancer mucin that recognize Tn antigen.
• Mouse intestinal goblet cells expressing SV40 T antigen directed by the MUC2 mucin gene promoter undergo apoptosis upon migration to the villi.
• Optical design of the U7 undulator beamline at the Pohang Light Source.
• Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells.
• Phorbol 12-myristate 13-acetate up-regulates the transcription of MUC2 intestinal mucin via Ras, ERK, and NF-kappa B.
• Stent-graft placement in a traumatic internal carotid-internal jugular fistula and pseudoaneurysm.
• Stereotactic aspiration of intracerebral haematoma: significance of surgical timing and haematoma volume reduction.
• Study of the aging perception.
• The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42.