Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides.

Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides. Research Abstract Details 

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Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides. Abstract Text:

Matthew G Woll,Samuel H Gellman,

An amide bond has been replaced by a thioester in bovine pancreatic polypeptide (bPP) to allow rapid and reversible (dynamic) exchange of the alpha-helical segment with other thiols in solution. We have begun to study the higher order structural stability of bPP by measuring the equilibrium constant of the "backbone thioester exchange" (BTE) reaction. The extent to which the equilibrium (KBTE) favors one set of peptides over the other, which can be easily measured, can be directly correlated to the energy gained from favorable noncovalent interactions that occur between peptide segments on either side of the thioester bond (Kfold).

Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides. Publishing Authors By Initials

MG Woll,SH Gellman,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

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Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 11172-4

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 15

MONTH: Sep

YEAR: 2004

Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides. Keywords Mesh Terms:

KEYWORDS: Tyrosine

MESH TERMS: chemistry

Chemical & Substance for Abstract: Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides. Information

Substance Name: Pancreatic Polypeptide

Registry Number: 59763-91-6

Grant and Affiliation Information for Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides.

AFFILIATION: Department of Chemistry, University of Wisconsin--Madison, 1101 University Avenue, Madison, Wisconsin 53706, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM61238

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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