B-Myb regulates the A(2B) adenosine receptor in vascular smooth muscle cells.

B-Myb regulates the A(2B) adenosine receptor in vascular smooth muscle cells. Research Abstract Details 

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B-Myb regulates the A(2B) adenosine receptor in vascular smooth muscle cells. Abstract Text:

Cynthia St Hilaire,Dan Yang,Barbara M Schreiber,Katya Ravid,Cynthia St Hilaire,Dan Yang,Barbara M Schreiber,Katya Ravid,

The A(2B) adenosine receptor (A(2B)AR) has been described to control various vascular functions, including inhibition of smooth muscle cell proliferation. Here, we sought to understand the regulation of A(2B)AR gene expression in aortic vascular smooth muscle cells (VSMCs), with a focus on the proliferation phase. Assays with A(2B)AR-beta-gal aortic VSMCs, in which the endogenous A(2B)AR gene promoter drives the expression of prokaryotic beta-galactosidase (beta-gal) instead of the endogenous A(2B)AR gene, show that beta-gal expression is upregulated when the cells are induced to exit from cell cycle arrest. Similarly, the level of A(2B)AR mRNA is upregulated in proliferating primary aortic VSMCs. In search of related mechanisms, it was noted that the A(2B)AR gene promoter contains several putative binding sites for the proliferation-inducing transcription factor, B-Myb. Using a clone of the 5' region upstream of the mouse A(2B)AR gene linked to a reporter gene, B-Myb site deletion mutants were generated. It was determined that B-Myb upregulates the A(2B)AR gene promoter, and specific promoter binding sites were identified as functional. In accordance, B-Myb also elevates endogenous A(2B)AR mRNA and receptor activity, and this activity decreases cell proliferation. Our data are novel in that they show that this proliferation-inhibiting A(2B)AR is itself an inducible receptor regulated by B-Myb. J. Cell. Biochem. 103: 1962-1974, 2007. (c) 2007 Wiley-Liss, Inc.

B-Myb regulates the A(2B) adenosine receptor in vascular smooth muscle cells. Publishing Authors By Initials

C St Hilaire,D Yang,BM Schreiber,K Ravid,C St Hilaire,D Yang,BM Schreiber,K Ravid,

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B-Myb regulates the A(2B) adenosine receptor in vascular smooth muscle cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of cellular biochemistry

VOLUME: 103

Page Numbers: 1962-74

Journal Abbreviation: J. Cell. Biochem.

ISSN: 1097-4644

DAY: 15

MONTH: Apr

YEAR: 2008

B-Myb regulates the A(2B) adenosine receptor in vascular smooth muscle cells. Information

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LANGUAGE: eng

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AFFILIATION: Department of Biochemistry and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118.

Country: United States

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MEDLINETA: J Cell Biochem

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