Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells.

Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells. Abstract Text:

Mei Dong,Kishore Guda,Prashant R Nambiar,Masako Nakanishi,Alexander C Lichtler,Mitsuo Nishikawa,Charles Giardina,Daniel W Rosenberg,

Inbred mice differ dramatically in their sensitivity to the colon carcinogen, azoxymethane (AOM). Identifying genes associated with this differential susceptibility in mice may ultimately reveal molecular mechanisms responsible for colon carcinogenesis. A cDNA array approach was taken to study gene expression changes induced by AOM in the colons of sensitive (A/J) and resistant (AKR) mice. Among the genes represented on the array, pre-adipocyte factor 1 (Pref-1), associated previously with suppression of adipocyte differentiation, was induced specifically by AOM in the distal colons of sensitive A/J mice (5.4-fold). Reverse transcription-PCR followed by sequence analysis revealed the presence of four alternative splice variants of Pref-1 mRNA in the colon. The potential significance of Pref-1 in colon tumorigenesis was explored in colon cancer cells infected with a retroviral construct containing the major splice variant. Over-expression of Pref-1A in HT-29 cells led to a marked resistance to butyrate-induced differentiation and growth inhibition. Our data indicate that Pref-1, a protein that suppresses differentiation and promotes colonocyte growth, may account in part for the sensitivity of A/J mice to AOM-induced carcinogenesis. In addition, detection of Pref-1 in a human colon tumor cell line suggests that it may also participate in human colon tumorigenesis.

Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells. Publishing Authors By Initials

M Dong,K Guda,PR Nambiar,M Nakanishi,AC Lichtler,M Nishikawa,C Giardina,DW Rosenberg,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Carcinogenesis

VOLUME: 25

Page Numbers: 2239-46

Journal Abbreviation: Carcinogenesis

ISSN: 0143-3334

DAY: 5

MONTH: 08

YEAR: 2004

Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8008055

Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells. Keywords Mesh Terms:

KEYWORDS: Reverse Transcriptase Polymerase Chain R

MESH TERMS: physiology

Chemical & Substance for Abstract: Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells. Information

Substance Name: Caspase 3

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells.

AFFILIATION: The University of Connecticut Health Center, Center for Molecular Medicine, Farmington, Connecticut, USA.

Country: England

AGENCY: United States NCI

GRANT: CA 81428-01

ACRONYM: CA

MEDLINETA: Carcinogenesis

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Azoxymethane-induced pre-adipocyte factor 1 Pref-1 functions as a differentiation inhibitor in colonic epithelial cells Related Publications

• Aerophagia as the initial presenting symptom of a depressed patient.
• Association between oral malodour and periodontal disease-related parameters in a population of 71 Israelis.
• Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells.
• Deoxycholic acid promotes the growth of colonic aberrant crypt foci.
• Development and sexual dimorphism of the pituitary gland.
• Direct searches for dark matter: recent results.
• Editorial: On Horace's Odes and Being a Referee.
• Estimation and prediction with HIV-treatment interruption data.
• Gastrointestinal stromal tumor detected on Tc-99m red blood cell scintigraphy.
• How should gaps be treated in parsimony? A comparison of approaches using simulation.
• How well are we doing in caring for the patient with primary malignant brain tumor? Are we measuring the outcomes that truly matter? A commentary.
• Individual, neighborhood, and state-level predictors of smoking among US Black women: a multilevel analysis.
• Isolation and characterization of kidney-derived stem cells.
• Matrix metalloproteinase-mediated disruption of tight junction proteins in cerebral vessels is reversed by synthetic matrix metalloproteinase inhibitor in focal ischemia in rat.
• Medical myth: The usefulness of pelvic exam.
• Nitric oxide-induced adenosine inhibition of hippocampal synaptic transmission depends on adenosine kinase inhibition and is cyclic GMP independent.
• Nonspecific and specific diffusion channels in the outer membrane of Escherichia coli.
• PRELIMINARY EXPERIMENTS TO DETERMINE THE NATURE OF THE CHARGE CARRIERS IN THE ELECTRICAL CONDUCTIVITY OF WET PROTEIN CRYSTALS.
• Pituitary volume in treatment-naïve pediatric major depressive disorder.
• Possibilities of experimental verification of the validity of urethral pressure profilometry.
• Predictors of rebellious behavior in childhood parental drug use, peers, school environment, and child personality.
• Proceedings of the sixth international conference on breath odor*.
• Quantitative evaluation of the effect of propylene glycol on BBB permeability.
• Racial discrimination and the incidence of hypertension in US black women.
• Relation between neighborhood median housing value and hypertension risk among black women in the United States.
• Statin use and risk of colorectal cancer.
• Symptom correlates of posttraumatic stress disorder in clients with borderline personality disorder.
• The coupling of catalytically relevant conformational fluctuations in subtilisin BPN' to solution viscosity revealed by hydrogen isotope exchange and inhibitor binding.
• The glutamate transporter EAAT2 is transiently expressed in developing human cerebral white matter.
• Validation of physical activity instruments: Black Women's Health Study.