Autotransporter structure reveals intra-barrel cleavage followed by conformational changes.

Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Research Abstract Details 

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Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Abstract Text:

Travis J Barnard,Nathalie Dautin,Petra Lukacik,Harris D Bernstein,Susan K Buchanan,Travis J Barnard,Nathalie Dautin,Petra Lukacik,Harris D Bernstein,Susan K Buchanan,

Autotransporters are virulence factors produced by Gram-negative bacteria. They consist of two domains, an N-terminal 'passenger' domain and a C-terminal beta-domain. beta-domains form beta-barrel structures in the outer membrane while passenger domains are translocated into the extracellular space. In some autotransporters, the two domains are separated by proteolytic cleavage. Using X-ray crystallography, we solved the 2.7-A structure of the post-cleavage state of the beta-domain of EspP, an autotransporter produced by Escherichia coli strain O157:H7. The structure consists of a 12-stranded beta-barrel with the passenger domain-beta-domain cleavage junction located inside the barrel pore, approximately midway between the extracellular and periplasmic surfaces of the outer membrane. The structure reveals an unprecedented intra-barrel cleavage mechanism and suggests that two conformational changes occur in the beta-domain after cleavage, one conferring increased stability on the beta-domain and another restricting access to the barrel pore.

Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Publishing Authors By Initials

TJ Barnard,N Dautin,P Lukacik,HD Bernstein,SK Buchanan,TJ Barnard,N Dautin,P Lukacik,HD Bernstein,SK Buchanan,

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Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Nature structural & molecular biology

VOLUME: 14

Page Numbers: 1214-20

Journal Abbreviation: Nat. Struct. Mol. Biol.

ISSN: 1545-9985

DAY: 11

MONTH: 11

YEAR: 2007

Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Autotransporter structure reveals intra-barrel cleavage followed by conformational changes.

AFFILIATION: Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, US National Institutes of Health, Bethesda, Maryland 20892, USA.

Country: United States

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MEDLINETA: Nat Struct Mol Biol

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ACCESSION NUMBER: 2QOM

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