Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia.

Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia. Research Abstract Details 

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Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia. Abstract Text:

Seema Khurana,Alok Tomar,Sudeep P George,Yaohong Wang,Mohammad Rizwan Siddiqui,Huazhang Guo,Gabor Tigyi,Sijo Mathew,

Autotaxin (ATX) is a potent tumor cell motogen that can produce lysophosphatidic acid (LPA) from lysophosphatidylcholine. LPA is a lipid mediator that has also been shown to modulate tumor cell invasion. Autotaxin mRNA is expressed at significant levels in the intestine. Likewise, LPA(2) receptor levels have been shown to be elevated in colon cancers. The molecular mechanism of ATX/LPA-induced increase in intestinal cell migration however, remains poorly understood. Villin is an intestinal and renal epithelial cell specific actin regulatory protein that modifies epithelial cell migration. In this study we demonstrate that both Caco-2 (endogenous villin) and MDCK (exogenous villin) cells, which express primarily LPA(2) receptors, show enhanced cell migration in response to ATX/LPA. ATX and LPA treatment results in the rapid formation of lamellipodia and redistribution of villin to these cell surface structures, suggesting a role for villin in regulating this initial event of cell locomotion. The LPA-induced increase in cell migration required activation of c-src kinase and downstream tyrosine phosphorylation of villin by c-src kinase. LPA stimulated cell motility was determined to be insensitive to pertussis toxin, but was regulated by activation of PLC-gamma(1). Together, our results show that in epithelial cells ATX and LPA act as strong stimulators of cell migration by recruiting PLC-gamma(1) and villin, both of which participate in the initiation of protrusion.

Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia. Publishing Authors By Initials

S Khurana,A Tomar,SP George,Y Wang,MR Siddiqui,H Guo,G Tigyi,S Mathew,

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Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Experimental cell research

VOLUME: 314

Page Numbers: 530-42

Journal Abbreviation: Exp. Cell Res.

ISSN: 0014-4827

DAY: 12

MONTH: 11

YEAR: 2007

Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia. Information

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LANGUAGE: eng

NlmUniqueID: 373226

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Grant and Affiliation Information for Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia.

AFFILIATION: Department of Physiology, University of Tennessee Health Science Center Memphis, Tennessee 38163, USA.

Country: United States

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MEDLINETA: Exp Cell Res

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