Autophagy in the heart and liver during normal aging and calorie restriction.

Autophagy in the heart and liver during normal aging and calorie restriction. Research Abstract Details 

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Autophagy in the heart and liver during normal aging and calorie restriction. Abstract Text:

Stephanie E Wohlgemuth,David Julian,Debora E Akin,Joanna Fried,Kristin Toscano,Christiaan Leeuwenburgh,William A Dunn,

Autophagy is a highly regulated intracellular process for the degradation of cellular constituents and essential for the maintenance of a healthy cell. We evaluated the effects of age and life-long calorie restriction on autophagy in heart and liver of young (6 months) and old (26 months) Fisher 344 rats. We observed that the occurrence of autophagic vacuoles was higher in heart than liver. The occurrence of autophagic vacuoles was not affected by age in either tissue, but was increased with calorie restriction in heart but not in liver. Next, we examined the expression of proteins involved in the formation and maturation of autophagosomes (beclin-1, LC3, Atg7, Atg9) or associated with autolysosomes and lysosomes (LAMP-1; cathepsin D). In hearts of both ad libitum-fed and calorie-restricted rats, we observed an increase in expression of beclin-1 and procathepsin D, but not mature cathepsin D, and a decrease in expression of LAMP-1 because of aging. In hearts, calorie restriction stimulated the expression of Atg7 and Atg9 and the lipidation of Atg8 (elevated LC3-II/I ratios) in aged rats. In hearts of ad libitum-fed rats, expression of Atg7 and lipidation of Atg8 were unaffected by age, while the cellular levels of Atg9 were lower in aged animals. Furthermore, we observed that the age- and diet-dependent expression levels of those proteins differed between heart and liver. In conclusion, autophagy in heart and liver did not decrease with age in ad libitum-fed rats, but was enhanced by calorie restriction in the heart. Thus, calorie restriction may mediate some of its beneficial effects by stimulating autophagy in the heart, indicating the potential for cardioprotective therapies.

Autophagy in the heart and liver during normal aging and calorie restriction. Publishing Authors By Initials

SE Wohlgemuth,D Julian,DE Akin,J Fried,K Toscano,C Leeuwenburgh,WA Dunn,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred f344 research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred f344 research

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Autophagy in the heart and liver during normal aging and calorie restriction. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Rejuvenation research

VOLUME: 10

Page Numbers: 281-92

Journal Abbreviation: Rejuvenation Res

ISSN: 1549-1684

DAY: 3

MONTH: Sep

YEAR: 2007

Autophagy in the heart and liver during normal aging and calorie restriction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101213381

Autophagy in the heart and liver during normal aging and calorie restriction. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred F344

MESH TERMS: cytology

Chemical & Substance for Abstract: Autophagy in the heart and liver during normal aging and calorie restriction. Information

Substance Name: Cathepsin D

Registry Number: EC 3.4.23.5

Grant and Affiliation Information for Autophagy in the heart and liver during normal aging and calorie restriction.

AFFILIATION: Biochemistry of Aging Laboratory, Department of Aging and Geriatric Research, College of Medicine, Institute on Aging, University of Florida, Gainesville, Florida 32610-0107, USA. swohlgemuth@aging.ufl.edu

Country: United States

AGENCY: United States NCI

GRANT: CA 95552

ACRONYM: CA

MEDLINETA: Rejuvenation Res

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