Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system.

Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system. Research Abstract Details 

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Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system. Abstract Text:

H Ariga,M Naruto,H Kawano,H Nakano,

Of the several DNA fragments present in the human lung cancer gene, 1.1 and 2.0 kilobase (kb) fragments corresponding to the intron of this gene were hybridized to a half part of the 27 nucleotides perfect palindrome present in the initiation part of replication in simian virus 40 (SV40) DNA. These two fragments cloned in pBR322 had good template activity, and the initiation of DNA replication started from the region of these fragments in an in vitro system, in which the initiation of DNA replication occurs on cloned DNA containing SV40 origin of DNA replication as described previously. Furthermore, these two clones could replicate autonomously in nuclei of SV40 transformed Cos cells, producing SV40 T antigen constitutively when the clones were transfected into Cos cells. These results show that functional SV40 origin-like sequences are present in human genomes, and they can replicate autonomously within the cells which are producing SV40 T antigen.

Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system. Publishing Authors By Initials

H Ariga,M Naruto,H Kawano,H Nakano,

For similar genetic structures: templates, genetic research abstracts see: genetic structures: templates, genetic research

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Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 1103-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1986

Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system. Keywords Mesh Terms:

KEYWORDS: Templates, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system. Information

Substance Name: DNA Restriction Enzymes

Registry Number: EC 3.1.21.-

Grant and Affiliation Information for Autonomous replicating sequences from intron of human ras gene in a simian virus 40 T antigen dependent system.

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Country: JAPAN

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MEDLINETA: J Biochem

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