Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells.

Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells. Abstract Text:

Kazuhiro Iwahashi,Hirotaka Yoshioka,Eleanor W Low,John R McCarrey,Ryuzo Yanagimachi,Yukiko Yamazaki,

In mice, unique events regulating epigenetic programming (e.g., genomic imprinting) and replication state (mitosis versus meiosis) occur during fetal germ cell development. To determine whether these processes are autonomously programmed in fetal germ cells or are dependent upon ongoing instructive interactions with surrounding gonadal somatic cells, we isolated male and female germ cells at 13.5 days postcoitum (dpc) and maintained them in culture for 6 days, either alone or in the presence of feeder cells or gonadal somatic cells. We examined allele-specific DNA methylation in the imprinted H19 and Snrpn genes, and we also determined whether these cells remained mitotic or entered meiosis. Our results show that isolated male germ cells are able to establish a characteristic "paternal" methylation pattern at imprinted genes in the absence of any support from somatic cells. On the other hand, cultured female germ cells maintain a hypomethylated status at these loci, characteristic of the normal "maternal" methylation pattern in endogenous female germ cells before birth. Further, the surviving female germ cells entered first meiotic prophase and reached the pachytene stage, whereas male germ cells entered mitotic arrest. These results indicate that mechanisms controlling both epigenetic programming and replication state are autonomously regulated in fetal germ cells that have been exposed to the genital ridge prior to 13.5 dpc.

Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells. Publishing Authors By Initials

K Iwahashi,H Yoshioka,EW Low,JR McCarrey,R Yanagimachi,Y Yamazaki,

For similar reproductive and urinary physiology: reproduction: sexual development research abstracts see: reproductive and urinary physiology: reproduction: sexual development research

PUBMED ID PMID:

MEDLINE DATE:

Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biology of reproduction

VOLUME: 77

Page Numbers: 697-706

Journal Abbreviation: Biol. Reprod.

ISSN: 0006-3363

DAY: 5

MONTH: 07

YEAR: 2007

Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 207224

Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells. Keywords Mesh Terms:

KEYWORDS: Sexual Development

MESH TERMS: genetics

Chemical & Substance for Abstract: Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells. Information

Substance Name: DNA (Cytosine-5-)-Methyltransferase

Registry Number: EC 2.1.1.37

Grant and Affiliation Information for Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells.

AFFILIATION: Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96813, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20RR16467

ACRONYM: RR

MEDLINETA: Biol Reprod

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells Related Publications

• A novel point mutation in the gene encoding capillary morphogenesis protein 2 in a Japanese patient with juvenile hyaline fibromatosis.
• Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord.
• Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells.
• Characteristics of congestive heart failure accompanied by atrial fibrillation with special reference to tachycardia-induced cardiomyopathy.
• Characterization of Aspergillus oryzae aspartyl aminopeptidase expressed in Escherichia coli.
• Current opinion: safety evaluation of drug metabolites in development of pharmaceuticals.
• Direct observation of reduction of met- and ferrylmyoglobins in the hemoglobin-free perfused rat heart.
• Effects of 2,4-substituents of deuterohemin upon peroxidase functions. I. Preparation and some properties of artificial enzymes.
• Expressions and mechanical functions of alpha1-adrenoceptor subtypes in hamster ureter.
• Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide.
• Heparin-induced thrombocytopenia, anomalous laboratory findings lead to the correct diagnosis.
• Hypersensitivity pneumonitis caused by Penicillium citrinum, not enoki spores.
• Individual differences and the chemical senses.
• Involvement of human cytochrome P450 2B6 in the omega- and 4-hydroxylation of the anesthetic agent propofol.
• One-electron and two-electron reductions of acceptors by xanthine oxidase and xanthine dehydrogenase.
• Postmortem diagnosis of Fabry disease with acromegaly and a unique vasculopathy.
• Prediction of cardiac events in patients with dilated cardiomyopathy using 123I-BMIPP and 201Tl myocardial scintigraphy.
• Preparation of bovine milk xanthine oxidase as a dehydrogenase form.
• Purification and some properties of formate dehydrogenase.
• Quality improvement of environmental secondary electron detector signal using helium gas in variable pressure scanning electron microscopy.
• Quantitative messenger RNA expression of Toll-like receptors and interferon-alpha1 in gingivitis and periodontitis.
• Rapidly progressive fatal pneumococcal sepsis in adults: a report of two cases.
• Reactions of the oxyform of horseradish peroxidase.
• Relationship between periodontal infections and systemic disease.
• Steady-state kinetics of the catalase reaction in the presence of cyanide.
• Synthesis and inhibitory activity of 4-alkynyl and 4-alkenylquinazolines: identification of new scaffolds for potent EGFR tyrosine kinase inhibitors.
• The 2-electron reduction of sperm whale ferryl myoglobin by ethanol.
• The effect of K+ concentration on the energy metabolism in perfused rat heart.
• The reaction of carbon monoxide with myoglobin in solution, in an amorphous state, and in crystals.
• Transient trimethylaminuria related to menstruation.