Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements.

Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. Research Abstract Details 

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Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. Abstract Text:

T Ohtsuki,R Vinayak,Y Watanabe,K Kita,G Kawai,K Watanabe,

RNA samples corresponding to Ascaris suum mitochondrial tRNA(Met) were chemically and automatically synthesized in amounts sufficient for NMR measurement. Conventional and rapid deprotection methods gave tRNA samples with the same amino acid-accepting activity as those prepared by other method; enzymatic synthesis, and enzymatic ligation of chemically synthesized fragments. The synthetic tRNA showed the same 1H-NMR spectrum in the iminoproton region as the ligated tRNA. This rapid and reliable preparation method thus provides biologically active tRNA for NMR measurement, and further, it is applicable for synthesis of other large synthetic RNAs, by combining the site-specific isotopic labeling method.

Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. Publishing Authors By Initials

T Ohtsuki,R Vinayak,Y Watanabe,K Kita,G Kawai,K Watanabe,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, transfer: rna, transfer, amino acid-specific: rna, transfer, met research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, transfer: rna, transfer, amino acid-specific: rna, transfer, met research

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Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 120

Page Numbers: 1070-3

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1996

Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. Keywords Mesh Terms:

KEYWORDS: RNA, Transfer, Met

MESH TERMS: chemistry

Chemical & Substance for Abstract: Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements.

AFFILIATION: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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