Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer.

Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. Research Abstract Details 

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Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. Abstract Text:

PURPOSE: Our purpose was to evaluate the effect of coculture on preembryo development and clinical outcome. METHODS: Enrolled patients underwent a luteal-phase endometrial biopsy. The tissue was then enzymatically digested (collagenase) and the stromal and glandular cells were separated by differential sedimentation rates. These cells were cultured to confluence, released, and then cryopreserved until the patient's in vitro fertilization (IVF)-embryo transfer (ET) cycle. All normally fertilized oocytes were then placed on the co-cultured cells until transfer on day 3. Preembryo development on co-culture was compared to that in the patient's noncocultured previous cycle. Implantation and clinical pregnancy rates were compared to those in a control group of patients undergoing IVF during the study period who were matched for age, stimulation protocol, number of oocytes retrieved, and preembryos transferred. RESULTS: Twenty-nine women underwent 31 cycles of IVF-ET. On day 3 the overall mean number of blastomeres per preembryo on co-culture compared to that in the patient's previous cycle was 6.3 +/- 1.8 vs. 5.6 +/- 1.2 (P = 0.04). The average percentage of cytoplasmic fragments on co-culture compared to the previous cycle was 16 +/- 9% vs. 19 +/- 9% (P = 0.32). At transfer, after preembryo selection, the mean number of blastomeres per preembryo on co-culture compared to that in the patient's previous cycle was 6.8 +/- 1.6 vs. 6.6 +/- 1.3 (P = 0.5). The implantation and clinical pregnancy rates between co-culture and the matched control group were 15% (14/93) vs. 13% (16/124) (P = 0.79) and 29% (9/31) vs. 25% (10/40) (P = 0.45). CONCLUSIONS: There was a significant improvement in the average number of blastomeres per preembryo on co-culture compared to that in the patient's previous noncoculture cycle. The overall implantation and clinical pregnancy rates between co-culture and a matched control group were not significantly different.

Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. Publishing Authors By Initials

For similar cells: connective tissue cells: stromal cells research abstracts see: cells: connective tissue cells: stromal cells research

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Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 16

Page Numbers: 121-7

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Mar

YEAR: 1999

Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. Keywords Mesh Terms:

KEYWORDS: Stromal Cells

MESH TERMS: physiology

Chemical & Substance for Abstract: Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. Information

Substance Name: Estradiol

Registry Number: 50-28-2

Grant and Affiliation Information for Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer.

AFFILIATION: Center for Reproductive Medicine and Infertility, New York Hospital-Cornell Medical Center, New York, New York, USA.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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