Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation.

Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation. Abstract Text:

Heng Chen,Heng Chen,

In order to decrease salt crystal formation, reduce the aggregation of viruses in atomic force microscopy (AFM) preparations and obtain an acceptable AFM image, the AFM sampling of viruses must be optimized. In this paper, centrifugal AFM sampling of recombinant adeno-associated virus-2 (rAAV-2) was used. The prepared rAAV-2 virus was imaged by AFM using the tapping mode in air. The results indicate that the rAAV-2 viruses prepared by centrifugal AFM sampling methods could be well imaged by AFM. The rAAV-2 viruses exhibited polymorphous particles in the AFM images. The preliminary off-line section analysis of the individual rAAV-2 virus particle indicated that the half-high widths of the large rAAV-2 particles ranged from 18 to 23 nm, while those of the small rAAV-2 particles ranged from 15 to 17 nm, which is almost in agreement with the results obtained by the off-line particle analysis of the rAAV-2 virus particles. Above all, the aggregation of different-sized rAAV-2 particles was directly imaged by AFM, which verifies the previous speculation that the large number (> ~31 nm) distribution of the mean diameter of rAAV-2 virus particles was probably caused by aggregation of rAAV-2 particles.

Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation. Publishing Authors By Initials

H Chen,H Chen,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Scanning

VOLUME: 29

Page Numbers: 238-42

Journal Abbreviation:

ISSN: 0161-0457

DAY: 15

MONTH: 10

YEAR: 2007

Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7903371

Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation.

AFFILIATION: College of Life Sciences and Biotechnology, Shanghai Jiaotong Univeristy, Shanghai, China. hengc@ualberta.ca

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Scanning

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Atomic force microscopy of recombinant adeno-associated virus-2 prepared by centrifugation Related Publications

• A new function of BMP4: dual role for BMP4 in regulation of Sonic hedgehog expression in the mouse tooth germ.
• An experimental study of anti-angiogenesis with recombinant human kringle 5.
• Application of Nd-Yag laser for gingivoplasty during orthodontic treatment.
• Clinical study of alveolar vertical distraction osteogenesis for implant.
• Enzyme-linked immunosorbent assay for the determination of p21-activated kinase activity.
• Hemorheology and expression of neutrophil adhesion molecules CD18 and CD62L in pancreatic microcirculation of Caerulein induced experimental acute pancreatitis.
• Lack of effect of dietary alpha-tocopherol on chemically induced hepatocarcinogenesis in rats.
• Prostaglandin E2 is involved in the increase of cytochrome P-450 2B1 expression by alpha-tocopheryl succinate in primary rat hepatocytes in the presence of phenobarbital.
• Rescue of cleft palate in Msx1-deficient mice by transgenic Bmp4 reveals a network of BMP and Shh signaling in the regulation of mammalian palatogenesis.
• Transgenically ectopic expression of Bmp4 to the Msx1 mutant dental mesenchyme restores downstream gene expression but represses Shh and Bmp2 in the enamel knot of wild type tooth germ.
• [A study on apoptosis and activity of SAPK/JNK in adrenocortical cells induced by cadmium chloride]
• [A study on central zone contour of tooth-crown vesticular surface among young people with normal occlusion]
• [Bone-inducing activity of human bone morphogenetic protein-2 102 peptide]
• [Comparative effects of tensiomin and sodium dimercaptosulphonate on the serum sulfydryl and anticopper of the patients with Wilson's disease]
• [Effects of fluoride on the expression of c-fos and c-jun genes and cell proliferation of rat osteoblasts]
• [Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping]
• [Experimental methods for mechanically stimulating the cells in vitro]
• [Focal adhesion kinase]
• [GATA-2 gene expression in leukemia patients and its significance]
• [Genetic polymorphisms of cytochrome P450 1A1 and susceptibility of early-onset Parkinson's disease]
• [Influence of chemotherapy on hematopoietic microenvironment and effect of autologous bone marrow stromal cell infusion on the recovery of hematopoiesis after chemotherapy]
• [Influence of the stratum corneum and dermis of the skin on the percutaneous absorption of fluorouracil delivered through skin patch]
• [Isolation, cultivation and identification of neural stem cell from human embryonic CNS]
• [Melanoma antigen-3 expression in human hepatocellular carcinoma]
• [Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction]
• [Preparation and application of a new chloromethylstyrene encapsulated silica single anion column]
• [Study on the intestinal microbial colonization resistance in patients with chronic severe hepatitis]
• [Synergetic transactivating functions of HCV core and truncated HBV middle surface protein]
• [Telomerase activity and cellular apoptosis of bladder cancers]
• [The apolipoprotein E gene polymorphism in children with steroid-resistant idiopathic nephrotic syndrome]