Suspension-derived protoplasts of Agropyron elongatum irradiated by ultra-violet light (UV) were fused with the suspension-derived protoplasts of Triticum astivum using PEG. Fertile intergeneric somatic hybrid plants were produced and various hybrid lines have been selected and propagated in successive generations. Their hybrid nature was confirmed by analysis of profiles of isozymes, RAPDs, and 5S rDNA spacer sequences, and via GISH analysis. By the procedure described, the phenotype and chromosome number of wheat could be maintained besides transfer of a few chromosomes and chromosomal fragments from the donor A. elongatum. The results above indicated that highly asymmetric fertile hybrid plants and hybrid progenies of wheat were produced via somatic hybridization.
Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Publishing Authors By Initials
Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Journal Published:
PUBLICATION TYPE: Research Support, Non-U.S. Gov
Journal: TAG. Theoretical and applied genetics. Theoretisch
VOLUME: 107
Page Numbers: 299-305
Journal Abbreviation:
ISSN: 0040-5752
DAY: 19
MONTH: 03
YEAR: 2003
Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Information
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LANGUAGE: eng
NlmUniqueID: 145600
Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Keywords Mesh Terms:
KEYWORDS: Ultraviolet Rays
MESH TERMS: physiology
Chemical & Substance for Abstract: Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Information
Substance Name: Isoenzymes
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Grant and Affiliation Information for Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi.
AFFILIATION: School of Life Sciences, Shandong University, Jinan 250100, P.R. China. Xiagm@life.sdu.edu.cn
Country: Germany
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MEDLINETA: Theor Appl Genet
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