Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin.

Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin. Abstract Text:

Kosuke Takeya,Masayuki Takahashi,Tsuyoshi Katoh,Michio Yazawa,

Although activities of smooth muscle myosin are regulated by phosphorylation, the molecular mechanisms of regulation have not been fully established. Phosphorylation of both heads of myosin is known to activate ATPase and motor activities, but the effects of phosphorylation of only one of the heads have not been established. Such information on singly phosphorylated myosin can serve to elucidate the molecular mechanism of the phosphorylation-dependent regulation. To understand the structural properties of the singly phosphorylated state, we prepared singly phosphorylated heavy meromyosin (HMM) containing a photoreactive benzophenone-labeled RLC and examined its photocross-linking reactivity. The two heads in the singly phosphorylated HMM showed different reactivities. The dephosphorylated RLC in the singly phosphorylated HMM was cross-linked to a heavy chain, like that in the dephosphorylated HMM, whereas the phosphorylated RLC did not react, like that in the fully phosphorylated HMM. These results indicate that the two heads of the singly phosphorylated HMM have an asymmetric structure, suggesting that phosphorylation of one head can to some extent activate smooth muscle HMM.

Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin. Publishing Authors By Initials

K Takeya,M Takahashi,T Katoh,M Yazawa,

For similar proteins: protein isoforms research abstracts see: proteins: protein isoforms research

PUBMED ID PMID:

MEDLINE DATE:

Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 138

Page Numbers: 245-53

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 2005

Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin. Keywords Mesh Terms:

KEYWORDS: Protein Isoforms

MESH TERMS: metabolism

Chemical & Substance for Abstract: Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin. Information

Substance Name: benzophenone

Registry Number: 119-61-9

Grant and Affiliation Information for Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin.

AFFILIATION: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin Related Publications

• Anti-hyperglycemic activity of kiwifruit leaf (Actinidia deliciosa) in mice.
• Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin.
• Biosynthesis of the blood group P antigen-like GalNAc beta 1-->3Gal beta 1-->4GlcNAc/Glc structure: a novel N-acetylgalactosaminyltransferase in human blood plasma.
• Characteristic thickened cell walls of the bracts of the 'eternal flower' Helichrysum bracteatum.
• Conformation, filament assembly, and activity of single-headed smooth muscle myosin.
• Conformations of vertebrate striated muscle myosin monomers in equilibrium with filaments.
• Crosslinking of telokin to chicken gizzard smooth muscle myosin.
• Electrocochleography in experimental endolymphatic hydrops.
• Essential light chain modulates phosphorylation-dependent regulation of smooth muscle myosin.
• Identification and characterization of a novel calcineurin-binding protein in scallop testis.
• Identification of trimeric myosin phosphatase (PP1M) as a target for a novel PKC-potentiated protein phosphatase-1 inhibitory protein (CPI17) in porcine aorta smooth muscle.
• Isolation and characterization of a subfragment of myosin A.
• Low molecular weight components (g-chains) of myosin from rabbit skeletal muscle. Separation, amino acid compositions and contents in myosin.
• Molecular cloning of cDNA encoding two subunits of calcineurin from scallop testis: demonstration of stage-specific expression during maturation of the testis.
• New Phospholipase A(1)-producing Bacteria from a Marine Fish.
• Properties of calcium-dependent regulatory proteins from fungi and yeast.
• Rebound phenomenon in glycerol test.
• Suppressive effects of Anoectochilus formosanus extract on osteoclast formation in vitro and bone resorption in vivo.
• Swimming test for evaluating vestibular function in guinea pigs.
• [Mediastinal parathyroid cyst]
• [Successful perioperative management with non-invasive positive pressure ventilation in muscular dystrophy with thymoma; report of a case]