Asymmetric localization of calpain 2 during neutrophil chemotaxis.

Asymmetric localization of calpain 2 during neutrophil chemotaxis. Research Abstract Details 

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Asymmetric localization of calpain 2 during neutrophil chemotaxis. Abstract Text:

Paul A Nuzzi,Melissa A Senetar,Anna Huttenlocher,

Chemoattractants induce neutrophil polarization through localized polymerization of F-actin at the leading edge. The suppression of rear and lateral protrusions is required for efficient chemotaxis and involves the temporal and spatial segregation of signaling molecules. We have previously shown that the intracellular calcium-dependent protease calpain is required for cell migration and is involved in regulating neutrophil chemotaxis. Here, we show that primary neutrophils and neutrophil-like HL-60 cells express both calpain 1 and calpain 2 and that chemoattractants induce the asymmetric recruitment of calpain 2, but not calpain 1, to the leading edge of polarized neutrophils and differentiated HL-60 cells. Using time-lapse microscopy, we show that enrichment of calpain 2 at the leading edge occurs during early pseudopod formation and that its localization is sensitive to changes in the chemotactic gradient. We demonstrate that calpain 2 is recruited to lipid rafts and that cholesterol depletion perturbs calpain 2 localization, suggesting that its enrichment at the front requires proper membrane organization. Finally, we show that catalytic activity of calpain is required to limit pseudopod formation in the direction of chemoattractant and for efficient chemotaxis. Together, our findings identify calpain 2 as a novel component of the frontness signal that promotes polarization during chemotaxis.

Asymmetric localization of calpain 2 during neutrophil chemotaxis. Publishing Authors By Initials

PA Nuzzi,MA Senetar,A Huttenlocher,

For similar proteins: cytoskeletal proteins: talin research abstracts see: proteins: cytoskeletal proteins: talin research

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Asymmetric localization of calpain 2 during neutrophil chemotaxis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 795-805

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 27

MONTH: 12

YEAR: 2006

Asymmetric localization of calpain 2 during neutrophil chemotaxis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Asymmetric localization of calpain 2 during neutrophil chemotaxis. Keywords Mesh Terms:

KEYWORDS: Talin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Asymmetric localization of calpain 2 during neutrophil chemotaxis. Information

Substance Name: calpain 2, human

Registry Number: EC 3.4.22.4

Grant and Affiliation Information for Asymmetric localization of calpain 2 during neutrophil chemotaxis.

AFFILIATION: Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM-074827

ACRONYM: GM

MEDLINETA: Mol Biol Cell

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