Association of leupaxin with Src in osteoclasts.

Association of leupaxin with Src in osteoclasts. Research Abstract Details 

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Association of leupaxin with Src in osteoclasts. Abstract Text:

Surasri Nandan Sahu,Mohammed Abdul Khadeer,Brian W Robertson,Stephanie M ,Guang Bai,Anandarup Gupta,

Leupaxin (LPXN), which belongs to the paxillin extended family of adaptor proteins, was previously identified as a component of the sealing zone in osteoclasts. LPXN was found to associate with several podosomal proteins, such as the protein tyrosine kinase Pyk2, the protein-tyrosine phosphatase-PEST (PTP-PEST), actin-binding proteins, and regulators of actin cytoskeletal reorganization. It was previously demonstrated that inhibition of LPXN expression resulted in reduced osteoclast-mediated resorption. In the current study, overexpression of LPXN in murine osteoclasts resulted in both enhanced resorptive activity and cell adhesion, as assessed by in vitro resorption assays. The overexpression of LPXN resulted in an increased association of Pyk2 with LPXN. In an attempt to determine an additional biochemical basis for the observed phenomenon in increased osteoclast activity, a coimmunoprecipitation screen for additional binding partners revealed that Src, a protein tyrosine kinase that is critical to both podosome formation and osteoclast function, was also associated with LPXN. After exposure to the pro-inflammatory and osteoclastogenic cytokine TNF-alpha, there was an increase in the level of Src that coimmunoprecipitated with LPXN. Our data indicate that association of the scaffold protein LPXN with Src adds further complexity to the organization of the podosomal signaling complex in osteoclasts.

Association of leupaxin with Src in osteoclasts. Publishing Authors By Initials

SN Sahu,MA Khadeer,BW Robertson,SM ,G Bai,A Gupta,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-tyrosine kinases: src-family kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-tyrosine kinases: src-family kinases research

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Association of leupaxin with Src in osteoclasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Cell physiology

VOLUME: 292

Page Numbers: C581-90

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 16

MONTH: 08

YEAR: 2006

Association of leupaxin with Src in osteoclasts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

Association of leupaxin with Src in osteoclasts. Keywords Mesh Terms:

KEYWORDS: src-Family Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Association of leupaxin with Src in osteoclasts. Information

Substance Name: src-Family Kinases

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Association of leupaxin with Src in osteoclasts.

AFFILIATION: Department of Biomedical Sciences, 4G-29, Dental School, University of Maryland-Baltimore, 666 West Baltimore St., Baltimore, MD 21201, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: AR 44792

ACRONYM: AR

MEDLINETA: Am J Physiol Cell Physiol

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