Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology.

Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. Research Abstract Details 

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Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. Abstract Text:

R M Locklin,B L Riggs,K C Hicok,H F Horton,M C Byrne,S Khosla,

Marrow stromal cells can differentiate into osteoblasts, adipocytes, myoblasts, and chondrocytes. Bone morphogenetic protein 2 (BMP-2) is a potent stimulator of osteoblastic differentiation, and identification of the genes regulated by BMP-2 in these cells should provide insight into the mechanism(s) of osteoblastic differentiation. Thus, we used a conditionally immortalized human marrow stromal cell line (hMS) and a gene expression microarray containing probes for a total of 6800 genes to compare gene expression in control and BMP-2-treated cultures. A total of 51 genes showed a consistent change in messenger RNA (mRNA) frequency between two repeat experiments. Seventeen of these genes showed a change in expression of at least 3-fold in BMP-2-treated cultures over control cultures. These included nuclear binding factors (10 genes), signal transduction pathway genes (2 genes), molecular transport (1 gene), cell surface proteins (2 genes) and growth factors (2 genes). Of particular interest were four of the nuclear binding factor genes ID-1, ID-2, ID-3, and ID-4. These encode dominant negative helix-loop-helix (dnHLH) proteins that lack the nuclear binding domain of the basic HLH proteins and thus have no transcriptional activity. They have been implicated in blocking both myogenesis and adipogenesis. Other transcription factors up-regulated at least 3-fold by BMP-2 included Dlx-2, HES-1, STAT1, and JunB. The changes in these nuclear binding factor mRNA levels were confirmed by real-time reverse-transcriptase-polymerase chain reaction (RT-PCR). A further three transcription factors, core binding factor beta (CBFbeta), AREB6, and SOX4, showed changes in expression of between 2- and 3-fold with BMP-2 treatment. In summary, we have used a gene chip microarray to identify a number of BMP-2 responsive genes in hMS cells. Thus, these studies provide potential candidate genes that may induce osteoblastic differentiation or, in the case of the ID proteins, block differentiation along alternate pathways.

Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. Publishing Authors By Initials

RM Locklin,BL Riggs,KC Hicok,HF Horton,MC Byrne,S Khosla,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bone and mineral research : the officia

VOLUME: 16

Page Numbers: 2192-204

Journal Abbreviation: J. Bone Miner. Res.

ISSN: 0884-0431

DAY: 19

MONTH: Dec

YEAR: 2001

Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8610640

Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: drug effects

Chemical & Substance for Abstract: Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology.

AFFILIATION: Endocrine Research Unit, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA.

Country: United States

AGENCY: United States NIA

GRANT: AG-04875

ACRONYM: AG

MEDLINETA: J Bone Miner Res

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