Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors.

Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors. Research Abstract Details 

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Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors. Abstract Text:

BACKGROUND: The clinical use of G-CSF has recently been expanded to include mobilization of stem cells for both autologous and allogeneic transplantation. Most of the published studies have focused on stem cells released into the peripheral blood (PB) after G-CSF treatment. However, little is known about the effects of G-CSF on BM. This study evaluated the concurrent effects of short-term G-CSF on both BM and PB stem and progenitor cells in normal individuals. METHODS: Volunteers received 5 or 10 microg/kg of G-CSF for 5 consecutive days (Days 1-5). On Days 0, 3, 6, 9 and 15, BM and PB samples were obtained. Flow cytometry and functional assay were performed to analyze stem cells, subpopulations, adhesion molecules, colony-forming units and LTCIC. RESULTS: The total nucleated cells and absolute numbers of CD34(+)/mL showed a similar response pattern in both BM and PB, with a peak around Day 6 that returned to baseline levels by Day 15. However, there was a reciprocal change in the percentage of CD34(+) cells between BM and PB compartments. The expressions of adhesion molecule showed an up- and down-regulation of alpha4 and alpha5 integrin subunits, respectively, also correlated with the CD34(+) mobilization patterns. DISCUSSIONS: The functional characterization of integrins, and further clinical examination of G-CSF-stimulated BM is warranted. G-CSF-stimulated BM maybe considered as an alternative source of stem cells in transplantation.

Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors. Publishing Authors By Initials

For similar persons: tissue donors research abstracts see: persons: tissue donors research

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Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cytotherapy

VOLUME: 4

Page Numbers: 55-63

Journal Abbreviation: Cytotherapy

ISSN: 1465-3249

DAY: 24

MONTH: 04

YEAR: 2002

Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors. Information

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LANGUAGE: eng

NlmUniqueID: 100895309

Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors. Keywords Mesh Terms:

KEYWORDS: Tissue Donors

MESH TERMS: immunology

Chemical & Substance for Abstract: Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors. Information

Substance Name: Granulocyte Colony-Stimulating Factor

Registry Number: 143011-72-7

Grant and Affiliation Information for Assessment of G-CSF stimulated BM hematopoietic stem cells in normal donors.

AFFILIATION: Division of Transplantation Medicine, Palmetto Richland Memorial Hospital, University of South Carolina, Columbia, SC, USA.

Country: England

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MEDLINETA: Cytotherapy

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