Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV.

Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV. Research Abstract Details 

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Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV. Abstract Text:

Christopher R Booth,Joanita Jakana,Wah Chiu,

CCD cameras have numerous advantages over photographic film for detecting electrons; however the point spread function of these cameras has not been sufficient for single particle data collection to subnanometer resolution with 300kV microscopes. We have adopted spectral signal to noise ratio (SNR) as a parameter for assessing detector quality for single particle imaging. The robustness of this parameter is confirmed under a variety of experimental conditions. Using this parameter, we demonstrate that the SNR of images of either amorphous carbon film or ice embedded virus particles collected on a new commercially available 4kx4k CCD camera are slightly better than photographic film at low spatial frequency (<1/5 Nyquist frequency), and as good as photographic film out to half of the Nyquist frequency. In addition it is slightly easier to visualize ice embedded particles on this CCD camera than on photographic film. Based on this analysis it is realistic to collect images containing subnanometer resolution data (6-9A) using this CCD camera at an effective magnification of approximately 112000x on a 300kV electron microscope.

Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV. Publishing Authors By Initials

CR Booth,J Jakana,W Chiu,

For similar investigative techniques: epidemiologic methods: epidemiologic research design: reproducibility of results research abstracts see: investigative techniques: epidemiologic methods: epidemiologic research design: reproducibility of results research

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Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of structural biology

VOLUME: 156

Page Numbers: 556-63

Journal Abbreviation: J. Struct. Biol.

ISSN: 1047-8477

DAY: 22

MONTH: 09

YEAR: 2006

Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV. Information

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LANGUAGE: eng

NlmUniqueID: 9011206

Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV. Keywords Mesh Terms:

KEYWORDS: Reproducibility of Results

MESH TERMS: methods

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Grant and Affiliation Information for Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV.

AFFILIATION: National Center For Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41RR02250

ACRONYM: RR

MEDLINETA: J Struct Biol

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