Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection.

Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. Research Abstract Details 

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Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. Abstract Text:

Celeste Bolin,Fernando Cardozo-Pelaez,

Oxidation of the guanosine moiety in DNA has become a hallmark biomarker in assessing oxidative stress. The oxidation of guanosine in the nucleotide triphosphate pool has been overlooked due to the lack of a reliable methodology. This method describes a sample processing and high performance liquid chromatography with electrochemical detection protocol for the analysis of the cellular pool of guanosine triphosphates and oxidized guanosine triphosphates. Validation of this method is demonstrated along with evaluation of these analytes in control and oxidizing conditions in vitro and in HEK 293T cells. Oxidation of this triphosphate pool occurred independently of oxidation to DNA.

Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. Publishing Authors By Initials

C Bolin,F Cardozo-Pelaez,

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Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Journal of chromatography. B, Analytical technolog

VOLUME: 856

Page Numbers: 121-30

Journal Abbreviation: J. Chromatogr. B Analyt. Techn

ISSN: 1570-0232

DAY: 2

MONTH: 06

YEAR: 2007

Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. Information

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LANGUAGE: eng

NlmUniqueID: 101139554

Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. Keywords Mesh Terms:

KEYWORDS: Reference Standards

MESH TERMS: analysis

Chemical & Substance for Abstract: Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. Information

Substance Name: Guanosine Triphosphate

Registry Number: 86-01-1

Grant and Affiliation Information for Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection.

AFFILIATION: Center for Environmental Health Sciences, Department of Pharmaceutical Sciences, University of Montana, Missoula, MT 59812, USA.

Country: Netherlands

AGENCY: United States NIA

GRANT: R15 AG023604-01

ACRONYM: AG

MEDLINETA: J Chromatogr B Analyt Technol

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