Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits.

Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits. Research Abstract Details 

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Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits. Abstract Text:

Y Okada,T Ohno,Y Nonomura,

The in vitro assembly reaction of tobacco mosaic virus (TMV), especially the elongation process of partially reconstituted RNA (PRR) by protein subunits, was observed by electron microscopy. After addition of TMV-protein subunits, the PRR appeared as rods with a clump at one end, believed to be a complex between added protein subunits and the RNA tail protruding from PRR. The subunits entrapped on the RNA tails in the forms of clumps were progressively incorporated into the growing rods on incubation, ending with the formation of completely reconstituted rods. The clumps were also observed after addition of cucumber green mottle mosaic virus (CGMMV) protein subunits to rods partially reconstituted from RNA and TMV-protein. In this case, the protein subunits, seen as clumps, did not become incorporated to form elongating rods. An improved model for the elongation of TMV rods is proposed. The elongation process is composed of two steps, with the first step being the interaction of protein subunits with the RNA tail protruding from the growing rod. Any protein having a specific binding site for TMV-rna, not limited to TMV-protein, will react in the first step. The second step is the incorporation of the protein on the RNA tail into a rod-shaped structure, with consequent elongation of the growing rod. It appears that only protein homologous with that in the partially reconstituted rods can partake in the second step.

Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits. Publishing Authors By Initials

Y Okada,T Ohno,Y Nonomura,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 77

Page Numbers: 1157-63

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1975

Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits. Information

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LANGUAGE: eng

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Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Assembly of tobacco mosaic virus in vitro. Improved model for the elongation process by protein subunits. Information

Substance Name: Viral Proteins

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Country: JAPAN

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MEDLINETA: J Biochem

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