Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes.

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Research Abstract Details 

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Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Abstract Text:

Marco van het Hoog,Timothy J Rast,Mikhail Martchenko,Suzanne Grindle,Daniel Dignard, Hogues,Christine Cuomo,Matthew Berriman,Stewart Scherer,B B Magee,Malcolm Whiteway,Hiroji Chibana, Nantel,P T Magee,

BACKGROUND: The 10.9x genomic sequence of Candida albicans, the most important human fungal pathogen, was published in 2004. Assembly 19 consisted of 412 supercontigs, of which 266 were a haploid set, since this fungus is diploid and contains an extensive degree of heterozygosity but lacks a complete sexual cycle. However, sequences of specific chromosomes were not determined. RESULTS: Supercontigs from Assembly 19 (183, representing 98.4% of the sequence) were assigned to individual chromosomes purified by pulse-field gel electrophoresis and hybridized to DNA microarrays. Nine Assembly 19 supercontigs were found to contain markers from two different chromosomes. Assembly 21 contains the sequence of each of the eight chromosomes and was determined using a synteny analysis with preliminary versions of the Candida dubliniensis genome assembly, bioinformatics, a sequence tagged site (STS) map of overlapping fosmid clones, and an optical map. The orientation and order of the contigs on each chromosome, repeat regions too large to be covered by a sequence run, such as the ribosomal DNA cluster and the major repeat sequence, and telomere placement were determined using the STS map. Sequence gaps were closed by PCR and sequencing of the products. The overall assembly was compared to an optical map; this identified some misassembled contigs and gave a size estimate for each chromosome. CONCLUSION: Assembly 21 reveals an ancient chromosome fusion, a number of small internal duplications followed by inversions, and a subtelomeric arrangement, including a new gene family, the TLO genes. Correlations of position with relatedness of gene families imply a novel method of dispersion. The sequence of the individual chromosomes of C. albicans raises interesting biological questions about gene family creation and dispersion, subtelomere organization, and chromosome evolution.

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Publishing Authors By Initials

M van het Hoog,TJ Rast,M Martchenko,S Grindle,D Dignard,H Hogues,C Cuomo,M Berriman,S Scherer,BB Magee,M Whiteway,H Chibana,A Nantel,PT Magee,

For similar proteins: dna-binding proteins: telomere-binding proteins research abstracts see: proteins: dna-binding proteins: telomere-binding proteins research

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Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genome biology

VOLUME: 8

Page Numbers: R52

Journal Abbreviation: Genome Biol.

ISSN: 1465-6914

DAY: 3

MONTH: 12

YEAR: 2007

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Information

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LANGUAGE: eng

NlmUniqueID: 100960660

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Keywords Mesh Terms:

KEYWORDS: Telomere-Binding Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Information

Substance Name: Telomere-Binding Proteins

Registry Number: 0

Grant and Affiliation Information for Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes.

AFFILIATION: Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada. marco@vanhethoog.com

Country: England

AGENCY: United States NIAID

GRANT: R01 AI 16567

ACRONYM: AI

MEDLINETA: Genome Biol

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