Assembly dynamics of Mycobacterium tuberculosis FtsZ.

Assembly dynamics of Mycobacterium tuberculosis FtsZ. Research Abstract Details 

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Assembly dynamics of Mycobacterium tuberculosis FtsZ. Abstract Text:

Yaodong Chen,David E Anderson,Malini Rajagopalan,Harold P Erickson,

We have investigated the assembly of FtsZ from Mycobacterium tuberculosis (MtbFtsZ). Electron microscopy confirmed the previous observation that MtbFtsZ assembled into long, two-stranded filaments at pH 6.5. However, we found that assembly at pH 7.2 or 7.7 produced predominantly short, one-stranded protofilaments, similar to those of Escherichia coli FtsZ (EcFtsZ). Near pH 7, which is close to the pH of M. tuberculosis cytoplasm, MtbFtsZ formed a mixture of single- and two-stranded filaments. We developed a fluorescence resonance energy transfer assay to measure the kinetics of initial assembly and the dynamic properties at steady state. Assembly of MtbFtsZ reached a plateau after 60-100 s, about 10 times slower than EcFtsZ. The initial assembly kinetics were similar at pH 6.5 and 7.7, despite the striking difference in the polymer structures. Both were fit with a cooperative assembly mechanism involving a weak dimer nucleus, similar to EcFtsZ but with slower kinetics. Subunit turnover and GTPase at steady state were also about 10 times slower for MtbFtsZ than for EcFtsZ. Specifically, the half-time for subunit turnover in vitro at pH 7.7 was 42 s for MtbFtsZ compared with 5.5 s for EcFtsZ. Photobleaching studies in vivo showed a range of turnover half-times with an average of 25 s for MtbFtsZ as compared with 9 s for EcFtsZ.

Assembly dynamics of Mycobacterium tuberculosis FtsZ. Publishing Authors By Initials

Y Chen,DE Anderson,M Rajagopalan,HP Erickson,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Assembly dynamics of Mycobacterium tuberculosis FtsZ. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 27736-43

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 20

MONTH: 07

YEAR: 2007

Assembly dynamics of Mycobacterium tuberculosis FtsZ. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Assembly dynamics of Mycobacterium tuberculosis FtsZ. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: chemistry

Chemical & Substance for Abstract: Assembly dynamics of Mycobacterium tuberculosis FtsZ. Information

Substance Name: Guanosine Triphosphate

Registry Number: 86-01-1

Grant and Affiliation Information for Assembly dynamics of Mycobacterium tuberculosis FtsZ.

AFFILIATION: Department of Cell Biology, Duke University, Medical Center, Durham, North Carolina 27710, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM66014

ACRONYM: GM

MEDLINETA: J Biol Chem

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