Assays for nonhomologous end joining in extracts.

Assays for nonhomologous end joining in extracts. Research Abstract Details 

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Assays for nonhomologous end joining in extracts. Abstract Text:

Joe Budman,Gilbert Chu,

In mammalian cells, nonhomologous end-joining (NHEJ) repairs DNA double strand breaks created by ionizing radiation and V(D)J recombination. Using human whole cell extracts prepared by the method of Baumann and West (1998), we have described a cell-free system for NHEJ that joins both compatible and noncompatible DNA ends (Budman and Chu, 2005). To measure joining efficiency and assess the processing of DNA ends, we developed a quantitative polymerase chain reaction assay for the joining of two specific DNA ends. The in vitro NHEJ reaction recapitulates key features of NHEJ observed in vivo: end joining is dependent on DNA-PK and XRCC4/Ligase4, and noncompatible ends are processed by polymerase and nuclease activities that often stabilize the alignment of opposing ends by base pairing. This chapter describes methods for preparing whole cell extracts and for studying the NHEJ reaction in vitro.

Assays for nonhomologous end joining in extracts. Publishing Authors By Initials

J Budman,G Chu,

For similar enzymes and coenzymes: enzymes: recombinases: vdj recombinases research abstracts see: enzymes and coenzymes: enzymes: recombinases: vdj recombinases research

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Assays for nonhomologous end joining in extracts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods in enzymology

VOLUME: 408

Page Numbers: 430-44

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 3

MONTH: 12

YEAR: 2006

Assays for nonhomologous end joining in extracts. Information

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LANGUAGE: eng

NlmUniqueID: 212271

Assays for nonhomologous end joining in extracts. Keywords Mesh Terms:

KEYWORDS: VDJ Recombinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Assays for nonhomologous end joining in extracts. Information

Substance Name: VDJ Recombinases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Assays for nonhomologous end joining in extracts.

AFFILIATION: Department of Medicine, Stanford University School of Medicine, California, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM58120

ACRONYM: GM

MEDLINETA: Methods Enzymol

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