Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles.

Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. Abstract Text:

Yasuhiro Aoyama,Takuya Kanamori,Takashi Nakai,Toshinori Sasaki,Shohei Horiuchi,Shinsuke Sando,Takuro Niidome,

Number- and size-controlled macromolecular associations are common in biology with viruses as a typical example. We report here a novel example of artificial viruses, in which the double-helical DNA is coated with 4-nm sized neutral glycocluster nanoparticles (GNPs) with a coating stoichiometry of approximately 2 GNPs per helical pitch (10 base pairs), where GNP arises from micellization of a cone-shaped, quadruple-chain glycocluster amphiphile having eight saccharide moieties with beta-glucoside termini on the calix[4]resorcarene macrocycle. The resulting "glycoviruses" are compactly packed (54 nm in the case of 7040 base-pair plasmid pCMVluc), are well charge-shielded (zeta congruent with approximately 0 mV), and effectively transfect cell cultures without notable cytotoxicity. The use of artificial viral vectors thus allows a new (nonamine/noncationic/nonpolymeric) access to gene delivery, a potential but still tough subject which has been studied extensively over the last 15 years by using viral or amine-based cationic vectors. The remarkable adhesion-manipulation ability of saccharide clusters also provides a strategy of bottom-up construction of nanometric or mesoscopic sizes.

Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. Publishing Authors By Initials

Y Aoyama,T Kanamori,T Nakai,T Sasaki,S Horiuchi,S Sando,T Niidome,

For similar viruses research abstracts see: viruses research

PUBMED ID PMID:

MEDLINE DATE:

Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 3455-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 26

MONTH: Mar

YEAR: 2003

Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. Keywords Mesh Terms:

KEYWORDS: Viruses

MESH TERMS: chemistry

Chemical & Substance for Abstract: Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles.

AFFILIATION: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. aoyamay@sbchem.kyoto-u.ac.jp

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Artificial viruses and their application to gene delivery Size-controlled gene coating with glycocluster nanoparticles Related Publications

• Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles.
• In vivo monitoring of intravenously injected gold nanorods using near-infrared light.
• Novel poly(ethylene glycol) derivatives with carboxylic acid pendant groups: synthesis and their protection and enhancing effect on non-viral gene transfection systems.