Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells.

Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells. Abstract Text:

Hui-Jun Zhou,Wei-Qin Wang,Guo-Dong Wu,Jun Lee,Ao Li,Hui-Jun Zhou,Wei-Qin Wang,Guo-Dong Wu,Jun Lee,Ao Li,

Artesunate (ART), a semi-synthetic derivative of artemisinin extracted from the Chinese herb Artemisia annua, is a safe and effective antimalarial drug. In the present investigation, we analyzed the inhibitory effects of ART on angiogenesis and on VEGF production in chronic myeloid leukemia (CML) K562 cells in vitro and in vivo. In order to analyze the effect of ART on VEGF secretion in K562 cells, we examined the level of VEGF secreted in conditioned media (CM) by ELISA assay. The result showed that ART could decrease the VEGF level in CM of K562 cells, even at a lower concentration (2 micromol/l, P<0.01). The inhibitory effect of in vitro angiogenesis was tested on aortic sprouting in fibrin gel. ART could effectively suppress the stimulating angiogenic ability of CM by pretreated with K562 cells for 48 h in a time-dependent manner (days 3-14). The antiangiogenic effect of ART was further evaluated in vivo in chicken chorioallantoic membrane (CAM) neovascularization model. The result indicated that the stimulating angiogenic activity was decreased in response to the K562 cells treated with ART or the CM from K562 cells pretreated with ART in a dose-dependent manner (3-12 micromol/l). Furthermore, we analyzed the level of VEGF expression by western blot and detected the form of VEGF mRNA by RT-PCR in K562 cells. The experiments showed that ART could inhibit the VEGF expression, correlated well with the level of VEGF secreted in CM. These findings suggest that ART might present potential antileukemia effect as a treatment for CML therapy, or as an adjunct to standard chemotherapeutic regimens.

Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells. Publishing Authors By Initials

HJ Zhou,WQ Wang,GD Wu,J Lee,A Li,HJ Zhou,WQ Wang,GD Wu,J Lee,A Li,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE: 2007 Aug-Sep

Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Vascular pharmacology

VOLUME: 47

Page Numbers: 131-8

Journal Abbreviation: Vascul. Pharmacol.

ISSN: 1537-1891

DAY: 24

MONTH: 05

YEAR: 2007

Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101130615

Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells.

AFFILIATION: Institute of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, PR China. zhouhj_zhouhj@yahoo.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Vascul Pharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Artesunate inhibits angiogenesis and downregulates vascular endothelial growth factor expression in chronic myeloid leukemia K562 cells Related Publications

• Airway epithelium and mucus: intracellular signaling pathways for gene expression and secretion.
• Imaging diagnosis of enlarged superior ophthalmic vein.
• Lung autotransplantation technique for treating central lung cancer of upper lobe.
• Study on the relationship between opioid receptors and fetal distress.
• The clinical value of rearrangement of IgH gene and bcl-2/J(H) fuse gene in the diagnosis of orbital lymphoproliferative disorders.
• The effect of metal copper on fracture healing in chicken.
• Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription.
• [Advances in research on ion-channel gating mechanism]
• [Association of dendritic cell infiltration with prognosis and lymph node metastasis of renal cell carcinoma]
• [Changes in serum concentration of podophyllotoxin after its topical application with liposome on rat skin]
• [ECG signal compression based on best basis of wavelet packets]
• [Effect of lovastatin on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro]
• [Effects of hypoxic exposure on coordinative expression of cytochrome oxidase subunits I and IV in rat cerebral cortex]
• [Effects of nitric oxide on pleural lymphatic stomata and lymphatic drainage in the rat]
• [Evaluation of p53, p21waf1, and PCNA in the diagnosis on lung cancer]
• [Evaluation the pathological diagnostic values of koilocyte in condyloma acuminatum]
• [Gap junctions in the nervous tissue]
• [Genotyping of HIV resistant alleles in indigenous Tibetan ethnic group of China: low frequency of CCR5triangle32 and high frequency of CCR2b-64I alleles]
• [Logistic regression analysis of wedge-shaped defects in the middle-aged and elderly]
• [Molecular genetic basis of Gilbert's syndrome]
• [Observation on the effect of disinfection to HBsAg by electrolyzed oxidizing water]
• [P2X purinoceptors and their regulations]
• [Preparation of rhBMP-2/BCB reconstituted bone xenograft and assay of its osteoinductivity]
• [Selective transmission of hepatitis B virus variant with S gene mutation between HBV-carrier mothers and their infants]
• [Surgical repair of postinfarction ventricular septal rupture]
• [Surgical treatment of anomalous origin of the left coronary artery from the pulmonary artery]
• [The correlations between plasma TNF-alpha, NO, NOS levels and brain lateralization in mice]
• [The genotype analysis of glucose-6-phosphate dehydrogenase deficiency in Yunnan province]
• [The relationship of low density lipoprotein receptor gene polymorphism and hyperlipidemia]
• [Umbilical cord blood transplantation (UCBT) in thalassemia children]