Arraying of intact liposomes into chemically functionalized microwells.

Arraying of intact liposomes into chemically functionalized microwells. Research Abstract Details 

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Arraying of intact liposomes into chemically functionalized microwells. Abstract Text:

Nikhil D Kalyankar,Manoj K Sharma,Shyam V Vaidya,David Calhoun,Charles Maldarelli,Alexander Couzis,Lane Gilchrist,

Here, we describe a protocol to bind individual, intact phospholipid bilayer liposomes, which are on the order of 1 microm in diameter, in microwells etched in a regular array on a silicon oxide substrate. The diameter of the wells is on the order of the liposome diameter, so only one liposome is located in each well. The background of the silicon oxide surface is functionalized with a PEG oligomer using the contact printing of a PEG silane to present a surface that resists the adsorption of proteins, lipid material, and liposomes. The interiors of the wells are functionalized with an aminosilane to facilitate the conjugation of biotin, which is then bound to Neutravidin. The avidin-coated well interiors bind the liposomes whose surfaces contain biotinylated lipids. The specific binding of the liposomes to the surface using the biotin-avidin linkage, together with the resistant nature of the background and the physical confinement of the wells, allows the liposomes to remain intact and to not unravel, rupture, and fuse onto the surface. We demonstrate this intact arraying using confocal laser scanning microscopy of fluorophores specifically tagging the microwells, the lipid bilayer, and the aqueous interior of the liposome.

Arraying of intact liposomes into chemically functionalized microwells. Publishing Authors By Initials

ND Kalyankar,MK Sharma,SV Vaidya,D Calhoun,C Maldarelli,A Couzis,L Gilchrist,

For similar inorganic chemicals: minerals: silicon dioxide research abstracts see: inorganic chemicals: minerals: silicon dioxide research

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Arraying of intact liposomes into chemically functionalized microwells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Langmuir : the ACS journal of surfaces and colloid

VOLUME: 22

Page Numbers: 5403-11

Journal Abbreviation:

ISSN: 0743-7463

DAY: 6

MONTH: Jun

YEAR: 2006

Arraying of intact liposomes into chemically functionalized microwells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9882736

Arraying of intact liposomes into chemically functionalized microwells. Keywords Mesh Terms:

KEYWORDS: Silicon Dioxide

MESH TERMS: chemistry

Chemical & Substance for Abstract: Arraying of intact liposomes into chemically functionalized microwells. Information

Substance Name: Silicon Dioxide

Registry Number: 7631-86-9

Grant and Affiliation Information for Arraying of intact liposomes into chemically functionalized microwells.

AFFILIATION: Department of Chemical Engineering, City University of New York, New York, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR03060

ACRONYM: RR

MEDLINETA: Langmuir

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