Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans.

Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans. Research Abstract Details 

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Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans. Abstract Text:

Ognian Bohorov,Hillevi Andersson-Sand,Julia Hoffmann,Ola Blixt,

Glycan array development is limited by the complexity of efficiently generating derivatives of free reducing glycans with primary amines or other functional groups. A novel bi-functional spacer with selective reactivity toward the free glycan and a second functionality, a primary amine, was synthesized. We demonstrated an efficient one-step derivatization of various glycans including naturally isolated N-glycans, O-glycans, milk oligosaccharides, and bacterial polysaccharides in microgram scale. No protecting group manipulations or activation of the anomeric center was required. To demonstrate its utility for glycan microarray fabrication, we compared glycans with different amine-spacers for incorporation onto an amine-reactive glass surface. Our study results revealed that glycans conjugated with this bi-functional linker were effectively printed and detected with various lectins and antibodies.

Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans. Publishing Authors By Initials

O Bohorov,H Andersson-Sand,J Hoffmann,O Blixt,

For similar carbohydrates: polysaccharides research abstracts see: carbohydrates: polysaccharides research

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Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Glycobiology

VOLUME: 16

Page Numbers: 21C-27C

Journal Abbreviation: Glycobiology

ISSN: 0959-6658

DAY: 12

MONTH: 09

YEAR: 2006

Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans. Information

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LANGUAGE: eng

NlmUniqueID: 9104124

Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans. Keywords Mesh Terms:

KEYWORDS: Polysaccharides

MESH TERMS: chemistry

Chemical & Substance for Abstract: Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans. Information

Substance Name: Polysaccharides

Registry Number: 0

Grant and Affiliation Information for Arraying glycomics: a novel bi-functional spacer for one-step microscale derivatization of free reducing glycans.

AFFILIATION: Glycan Array Synthesis Core D, Consortium for Functional Glycomics, Department of Molecular Biology, The Scripps Research Institute, CB 248A 10550 N, Torrey Pines Road, La Jolla, CA 92037, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM62116

ACRONYM: GM

MEDLINETA: Glycobiology

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