Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore.

Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Research Abstract Details 

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Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Abstract Text:

The Ndc80 complex is a constituent of the outer plate of the kinetochore and plays a critical role in establishing the stable kinetochore-microtubule interactions required for chromosome segregation in mitosis. The Ndc80 complex is evolutionarily conserved and contains the four subunits Spc24, Spc25, Nuf2, and Ndc80 (whose human homologue is called Hec1). All four subunits are predicted to contain globular domains and extensive coiled coil regions. To gain an insight into the organization of the human Ndc80 complex, we reconstituted it using recombinant methods. The hydrodynamic properties of the recombinant Ndc80 complex are identical to those of the endogenous HeLa cell complex and are consistent with a 1:1:1:1 stoichiometry of the four subunits and a very elongated shape. Two tight Hec1-Nuf2 and Spc24-Spc25 subcomplexes, each stabilized by a parallel heterodimeric coiled coil, maintain this organization. These subcomplexes tetramerize via an interaction of the C- and N-terminal portions of the Hec1-Nuf2 and Spc24-Spc25 coiled coils, respectively. The recombinant complex displays normal kinetochore localization upon injection in HeLa cells and is therefore a faithful copy of the endogenous Ndc80 complex.

Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Publishing Authors By Initials

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 280

Page Numbers: 29088-95

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 16

MONTH: 06

YEAR: 2005

Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Information

Substance Name: Glutathione Transferase

Registry Number: EC 2.5.1.18

Grant and Affiliation Information for Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore.

AFFILIATION: Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy.

Country: United States

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MEDLINETA: J Biol Chem

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