Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro.

Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro. Research Abstract Details 

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Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro. Abstract Text:

Y Sugiyama,Y Mukohata,

Archae-opsin-1 (aO-1) has been expressed efficiently as a fusion protein with 13 heterologous amino acids at the amino terminus of the mature aO-1 in Escherichia coli under the control of T7 promoter. The E. coli-expressed aO-1, designated as aO-1002, which was located in the membrane fraction, was extracted with 8 M urea and partially purified by gel filtration chromatography in the presence of SDS. When all-trans retinal was added, aO-1002 in dimyristoylphosphatidylcholine and detergent-mixed micelles was converted to a purple pigment with lambda max at 558 nm at 20 degrees C via a 435/460 nm intermediate. Conversion of the intermediate to purple pigment was the rate-limiting step and proceeded as a two-state transition, because an isosbestic point was seen at 485 nm. Similar spectral changes were also observed in the regeneration process of hydroxylamine-bleached claret membranes and aO-1 isolated from claret membranes. Thus, the polypeptide of aO-1002 is considered to fold and form a retinal binding pocket in phospholipid and detergent micelles similarly to aO-1 isolated from the halobacterial membranes. Purple pigment showed a light-driven proton-pumping activity when reconstituted into phosphatidylcholine liposomes.

Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro. Publishing Authors By Initials

Y Sugiyama,Y Mukohata,

For similar organic chemicals: aldehydes: retinaldehyde research abstracts see: organic chemicals: aldehydes: retinaldehyde research

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Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 115

Page Numbers: 1021-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1994

Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro. Keywords Mesh Terms:

KEYWORDS: Retinaldehyde

MESH TERMS: metabolism

Chemical & Substance for Abstract: Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro. Information

Substance Name: Retinaldehyde

Registry Number: 116-31-4

Grant and Affiliation Information for Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro.

AFFILIATION: Department of Biology, Faculty of Science, Nagoya University, Aichi.

Country: JAPAN

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MEDLINETA: J Biochem

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