Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei.

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Abstract Text:

Dominique Promeneur,Yangjian Liu,Jorge Maciel,Peter Agre,Landon S King,Nirbhay Kumar,

The malaria parasite can use host plasma glycerol for lipid biosynthesis and membrane biogenesis during the asexual intraerythrocytic development. The molecular basis for glycerol uptake into the parasite is undefined. We hypothesize that the Plasmodium aquaglyceroporin provides the pathway for glycerol uptake into the malaria parasite. To test this hypothesis, we identified the orthologue of Plasmodium falciparum aquaglyceroporin (PfAQP) in the rodent malaria parasite, Plasmodium berghei (PbAQP), and examined the biological role of PbAQP by performing a targeted deletion of the PbAQP gene. PbAQP and PfAQP are 62% identical in sequence. In contrast to the canonical NPA (Asn-Pro-Ala) motifs in most aquaporins, the PbAQP has NLA (Asn-Leu-Ala) and NPS (Asn-Leu-Ser) in those positions. PbAQP expressed in Xenopus oocytes was permeable to water and glycerol, suggesting that PbAQP is an aquaglyceroporin. In P. berghei, PbAQP was localized to the parasite plasma membrane. The PbAQP-null parasites were viable; however, they were highly deficient in glycerol transport. In addition, they proliferated more slowly compared with the WT parasites, and mice infected with PbAQP-null parasites survived longer. Taken together, these findings suggest that PbAQP provides the pathway for the entry of glycerol into P. berghei and contributes to the growth of the parasite during the asexual intraerythrocytic stages of infection. In conclusion, we demonstrate here that PbAQP plays an important role in the blood-stage development of the rodent malaria parasite during infection in mice and could be added to the list of targets for the design of antimalarial drugs.

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Publishing Authors By Initials

D Promeneur,Y Liu,J Maciel,P Agre,LS King,N Kumar,

For similar proteins: protozoan proteins research abstracts see: proteins: protozoan proteins research

PUBMED ID PMID:

MEDLINE DATE:

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 2211-6

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 6

MONTH: 02

YEAR: 2007

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Keywords Mesh Terms:

KEYWORDS: Protozoan Proteins

MESH TERMS: pathogenicity

Chemical & Substance for Abstract: Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Information

Substance Name: Glycerol

Registry Number: 56-81-5

Grant and Affiliation Information for Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei.

AFFILIATION: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2185, USA. d.promeneur@cellbio.duke.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL48268

ACRONYM: HL

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei Related Publications

• A modified Plasmodium falciparum growth inhibition assay (GIA) to assess activity of plasma from malaria endemic areas.
• A new paradigm for the treatment of high-risk prostate cancer: radiosensitization with docetaxel.
• An inherent role of microtubule network in the action of nuclear receptor.
• Antibody responses to Plasmodium falciparum vaccine candidate antigens in three areas distinct with respect to altitude.
• Breast cancer-amplified sequence 3, a target of metastasis-associated protein 1, contributes to tamoxifen resistance in premenopausal patients with breast cancer.
• Ciz1, a Novel DNA-binding coactivator of the estrogen receptor alpha, confers hypersensitivity to estrogen action.
• Cognitive function in adults with type 2 diabetes and major depression.
• Diagnosis and management of extensive vertex birth molding.
• Dynein light chain 1, a p21-activated kinase 1-interacting substrate, promotes cancerous phenotypes.
• Effect of individual and multiple antioxidant vitamins on growth and morphology of human nontumorigenic and tumorigenic parotid acinar cells in culture.
• Functional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro.
• HPA axis activity and neuropathogenesis in HIV-1 clade C infection.
• How substance use differs among American secondary schools.
• Influence of Agrobacterium rhizogenes Strains on Biomass and Alkaloid Productivity in Hairy Root Lines of Hyoscyamus muticus and H. albus *.
• Letter to the editor: The suckling-micturition reflex in cows.
• Metal-binding properties of human centrin-2 determined by micro-electrospray ionization mass spectrometry and UV spectroscopy.
• Metastasis tumor antigens, an emerging family of multifaceted master coregulators.
• Metastatic tumor antigen 3 is a direct corepressor of the Wnt4 pathway.
• Modification of the expression of adenosine 3',5'-cyclic monophosphate-induced differentiated functions in neuroblastoma cells by beta-carotene and D-alpha-tocopheryl succinate.
• P21-activated kinases in human cancer.
• Pituitary abscess: Report of four cases and review of literature.
• Potential medicinal plants for CNS disorders: an overview.
• Protein disulfide isomerase assisted protein folding in malaria parasites.
• Quality of life in HIV subtype C infection among asymptomatic subjects and its association with CD4 counts and viral loads--a study from South India.
• Re-engineering cytochrome P450 2B11dH for enhanced metabolism of several substrates including the anti-cancer prodrugs cyclophosphamide and ifosfamide.
• Sexual differentiation and development in the malaria parasite.
• Standard wound coverage techniques for extremity war injury.
• Systemic, cellular and molecular analysis of chemoreflex-mediated sympathoexcitation by chronic intermittent hypoxia.
• Variable region 4 of SIV envelope correlates with rapid disease progression in morphine-exposed macaques infected with SIV/SHIV.
• p21-activated kinase signaling in breast cancer.