Aptamers switch on fluorescence of triphenylmethane dyes.

Aptamers switch on fluorescence of triphenylmethane dyes. Research Abstract Details 

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Aptamers switch on fluorescence of triphenylmethane dyes. Abstract Text:

Jeremy R Babendure,Stephen R Adams,Roger Y Tsien,

MG and SRB aptamers, which are short RNA sequences originally selected only for binding to malachite green or sulforhodamine B, can greatly enhance the fluorescence of normally nonfluorescent triphenylmethane dyes. MG aptamer enhances the quantum yields of malachite green (MG) and a novel rigidized derivative, indolinyl malachite green (IMG) by >2000-fold. SRB aptamer brightens patent blue V and VF by >90-fold. These enhancements are specific because MG aptamer has no effect on patent blue dyes and SRB aptamer has little or no effect on MG and IMG. Such sequence-specific fluorescence labeling of short RNA motifs is a first step toward genetically encodable fusion tags for imaging selected RNAs in vitro and in cells.

Aptamers switch on fluorescence of triphenylmethane dyes. Publishing Authors By Initials

JR Babendure,SR Adams,RY Tsien,

For similar organic chemicals: amines: aniline compounds: rosaniline dyes research abstracts see: organic chemicals: amines: aniline compounds: rosaniline dyes research

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Aptamers switch on fluorescence of triphenylmethane dyes. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 14716-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 3

MONTH: Dec

YEAR: 2003

Aptamers switch on fluorescence of triphenylmethane dyes. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Aptamers switch on fluorescence of triphenylmethane dyes. Keywords Mesh Terms:

KEYWORDS: Rosaniline Dyes

MESH TERMS: chemistry

Chemical & Substance for Abstract: Aptamers switch on fluorescence of triphenylmethane dyes. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Aptamers switch on fluorescence of triphenylmethane dyes.

AFFILIATION: Department of Pharmacology, Howard Hughes Medical Institute, University of California-San Diego, La Jolla, CA 92093-0647, USA.

Country: United States

AGENCY: United States PHS

GRANT: NIH 27177

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MEDLINETA: J Am Chem Soc

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