Application of the hepatocytes bioreactor to xenobiotic biotransformation.

Application of the hepatocytes bioreactor to xenobiotic biotransformation. Research Abstract Details 

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Application of the hepatocytes bioreactor to xenobiotic biotransformation. Abstract Text:

L ,H Farghali,D ,D ,S Hynie,

This study deals with the application of the previously developed immobilized and perfused isolated hepatocytes as a cellular system for the study of representative phase I and phase II of biotransformation reactions. To illustrate phase I reactions, aminopyrine (0.17-4.25 mmol/l) and hexobarbital (0.2 mmol/l) were selected. For phase II reactions, glutathione transferase activity was evaluated by using 1-chloro-2,4-dinitrobenzene (CDNB) as a substrate (0.125-2.0 mmol/l). Formaldehyde, that was formed from aminopyrine, increased steadily in the perfusion medium with time. The perfused hepatocytes eliminated hexobarbital at a much higher rate than the hepatocytes in suspension. At several time points the amount of CDNB-glutathione conjugate formed per one million hepatocytes in the bioreactor was almost twice the amount formed by the hepatocytes in suspension. The present data illustrate the successful application of the hepatocyte bioreactor in phase I and phase II of xenobiotic metabolism and indicate that the cells were metabolically more active than the cells in suspension.

Application of the hepatocytes bioreactor to xenobiotic biotransformation. Publishing Authors By Initials

L ,H Farghali,D ,D ,S Hynie,

For similar pharmaceutical preparations: xenobiotics research abstracts see: pharmaceutical preparations: xenobiotics research

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Application of the hepatocytes bioreactor to xenobiotic biotransformation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Physiological research / Academia Scientiarum Bohe

VOLUME: 43

Page Numbers: 127-30

Journal Abbreviation: Physiol Res

ISSN: 0862-8408

DAY: 2

MONTH: 04

YEAR: 1994

Application of the hepatocytes bioreactor to xenobiotic biotransformation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9112413

Application of the hepatocytes bioreactor to xenobiotic biotransformation. Keywords Mesh Terms:

KEYWORDS: Xenobiotics

MESH TERMS: metabolism

Chemical & Substance for Abstract: Application of the hepatocytes bioreactor to xenobiotic biotransformation. Information

Substance Name: Glutathione Transferase

Registry Number: EC 2.5.1.18

Grant and Affiliation Information for Application of the hepatocytes bioreactor to xenobiotic biotransformation.

AFFILIATION: Institute of Pharmacology, First Medical Faculty, Charles University, Prague, Czech Republic.

Country: CZECH REPUBLIC

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MEDLINETA: Physiol Res

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