OBJECTIVE: To study the feasibility of application of rat-tail collagen in the primary culture of rats marginal cells of stria vascularis. METHOD: The effect of self-made rat-tail collagen on the primary culture of rats marginal cells of stria vascularis was observed and estimated. RESULT: When cochlear stria vascularis fragment was isolalted and cultured for 24 h, a few culture cells appeared around the fragments. About 48 to approximately 72 h later, clusters of culture cells could be seen,these cells showed the cobblestone shape under the microscope. The immunohistochemistry and the transmission electron microscopy showed the epithelial origination of these cells. CONCLUSION: It is feasible to the primarily culture the rats marginal cells of stria vascularis with self-made rat-tail collagen.
[Application of rat tail collagen in the primary culture of rats marginal cells of stria vascularis] Publishing Authors By Initials