Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets.

Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. Research Abstract Details 

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Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. Abstract Text:

Veronica D Gonzalez,Niklas K ,Karl-Johan Malmberg,Markus Moll,Carlotta Kuylenstierna,Jakob ,Hans-Gustaf Ljunggren,Johan K Sandberg,Veronica D Gonzalez,Niklas K ,Karl-Johan Malmberg,Markus Moll,Carlotta Kuylenstierna,Jakob ,Hans-Gustaf Ljunggren,Johan K Sandberg,

Innate and adaptive cellular immunity is initiated, directed and regulated by a vast array of cell surface receptors. Attempts to harness the cellular immune system in translational settings such as immunotherapy and vaccine development require tools to accurately describe and isolate lymphocytes with specific characteristics. One such tool, flow cytometry, is undergoing a revolution in instrumentation and reagents, providing opportunities for high resolution phenotypic and functional analysis of lymphocytes. Here, we demonstrate how nine-color flow cytometry can be adapted, optimized and applied to investigate the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. We provide examples of studies of adaptive T cell responses against viruses, as well as the assessment of CD1d-restricted NKT cells and NK cells. We discuss the importance of this technology for detailed investigations of lymphocyte subsets in studies of infectious diseases and cancer.

Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. Publishing Authors By Initials

VD Gonzalez,NK ,KJ Malmberg,M Moll,C Kuylenstierna,J ,HG Ljunggren,JK Sandberg,VD Gonzalez,NK ,KJ Malmberg,M Moll,C Kuylenstierna,J ,HG Ljunggren,JK Sandberg,

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Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of immunological methods

VOLUME: 330

Page Numbers: 64-74

Journal Abbreviation: J. Immunol. Methods

ISSN: 0022-1759

DAY: 4

MONTH: 12

YEAR: 2007

Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets.

AFFILIATION: Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Country: Netherlands

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MEDLINETA: J Immunol Methods

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