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Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts.

Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts. Research Abstract Details 

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  • Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts. Abstract Text:

    yun zhangYun Zhang,cuiping maoCuiping Mao,weimin yanWeimin Yan,xiaoxiang zhengXiaoxiang Zheng,

    To investigate application of cell engineering in herbal Medicine treating bone resorption. Study design: the bone marrow cells were isolated from 5 week-old SD rats and induced by 1,25(OH)2D3. Firstly, Morphological assay, cells were fixed, stained by tartrate-resistant acid phosphatase (TRAP) and observed with the fluorescence micrograph. F-actin rings of OCLs were labled using Bodipy FL Phallacidin and PI, and observed with the laser scanning confocal microscope, then also showed the ultrastructure of them with the scanning electron microscope. Secondly, bone pits were observed after bone slices were stained by paraosaniline hydrochloride. Thirdly, the number of TRAP (+) cells with above two nuclei was counted, and TRAP activity of OCLs was examined with TRAP kit. In addition, effects of Traditional Chinese Medicine, such as Oral Liquid of Guiluerxian, on the differentiation and function of OCLs were observed. Results indicated that application of cell engineering made it possible to observe vividly the morphology of osteoclasts, study its function and mechanism underlying bone resorption.

    Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts. Publishing Authors By Initials

    y zhangY Zhang,c maoC Mao,w yanW Yan,x zhengX Zheng,

    For similar abstracts research abstracts see: abstracts research

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    Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Conference proceedings : ... Annual International

    VOLUME: 5

    Page Numbers: 4958-61

    Journal Abbreviation:

    ISSN: 1557-170X

    DAY: 6

    MONTH: 02

    YEAR: 2005

    Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts. Information

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    LANGUAGE: eng

    NlmUniqueID: 101243413

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    Grant and Affiliation Information for Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts.

    AFFILIATION: Dept. of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang University, Zheda Road 38, 310027 Hangzhou, China.

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: Conf Proc IEEE Eng Med Biol So

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