Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults.

Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults. Abstract Text:

Itaru Sato,Filippo Barni,Miki Yoshiike,Cesare Rapone,Andrea Berti,Shinichi Nakaki,Kazuki Yamazaki,Fumio Ishikawa,Teruaki Iwamoto,Itaru Sato,Filippo Barni,Miki Yoshiike,Cesare Rapone,Andrea Berti,Shinichi Nakaki,Kazuki Yamazaki,Fumio Ishikawa,Teruaki Iwamoto,

A commercially available semen detection kit, Nanotrap Sg, which employs a one-step detection test based on immunochromatographic assay for the semenogelin protein, was evaluated for profiling male-specific DNA in sexual assault casework samples. While semen diluted with phosphate-buffered saline held and kept at 4 degrees C for 1 week showed a relatively strong signal intensity with Nanotrap Sg, the signal intensity was decreased by dilution after storage at 4 degrees C or freezing and thawing repeated more than three times. The reproducibility of Nanotrap Sg was tested on a total of 174 sexual assault casework samples from three forensic laboratories using intra- and interassay and no variation was observed in the semenogelin (Sg) signal. The positive signal ratio was 12.6% higher for prostate-specific antigen immunochromatographic membrane tests than Nanotrap Sg. Although spermatozoa were not confirmed in 61 (35%) out of 174 samples, Sg-positive signals could be detected from 41 (67%) of the 61 samples. Female genetic profiles could be observed in 95% of the samples, which tested negative for Sg on the Nanotrap Sg test, but no male genetic profiles could be observed. These results suggest that Nanotrap Sg can positively identify samples containing male DNA even in the absence of detectable intact spermatozoa. Further, Sg-positive signals identified samples for which male-specific DNA profiling could be performed, even if no sperm could be detected from the sample. The potential of Nanotrap Sg for identifying forensic samples with male-specific DNA was clearly demonstrated.

Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults. Publishing Authors By Initials

I Sato,F Barni,M Yoshiike,C Rapone,A Berti,S Nakaki,K Yamazaki,F Ishikawa,T Iwamoto,I Sato,F Barni,M Yoshiike,C Rapone,A Berti,S Nakaki,K Yamazaki,F Ishikawa,T Iwamoto,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: International journal of legal medicine

VOLUME: 121

Page Numbers: 315-9

Journal Abbreviation: Int. J. Legal Med.

ISSN: 0937-9827

DAY: 1

MONTH: 04

YEAR: 2006

Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9101456

Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults.

AFFILIATION: Scientific Crime Laboratory, Kanagawa Prefectural Police, 155-1 Yamashita-cho, Naka-ku, Yokohama 231-0023, Japan. itaru-s@m2.ocv.ne.jp

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Int J Legal Med

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults Related Publications

• A study to determine the effects of food and multiple dosing on the pharmacokinetics of vorinostat given orally to patients with advanced cancer.
• Age-related differences in muscle activity, stride frequency and heart rate response during walking in water.
• Applicability of Nanotrap Sg as a semen detection kit before male-specific DNA profiling in sexual assaults.
• Comparative analysis of interindividual variations in the seminal plasma proteome of fertile men with identification of potential markers for azoospermia in infertile patients.
• Computed tomography-based navigation to determine the socket location in total hip arthroplasty of an osteoarthritis hip with a large leg length discrepancy due to severe acetabular dysplasia.
• Deep gray matter hyperperfusion with occipital hypoperfusion in dementia with Lewy bodies.
• Desmoplastic fibroblastoma (collagenous fibroma) with a specific breakpoint of 11q12.
• Detection of luminal contour using fuzzy clustering and mathematical morphology in intravascular ultrasound images.
• Difference in segmental lean and fat mass components between pre- and postmenopausal women.
• Etiology of decreased gastric nitric oxide in the critically ill.
• Exercise training after maturation induces neuroplastic changes in cardiorespiratory and locomotor centers of the brain: 1609: board #99 may 30 3:30 PM - 5:00 PM.
• Extremely low birth weight infants are at high risk for auditory neuropathy.
• Factors associated with pain and functional limitation in Japanese male patients with knee osteoarthritis.
• Hedgehog proteins stimulate chondrogenic cell differentiation and cartilage formation.
• Impact of neonatal hypothyroidism on reproduction in the male hamster, Mesocricetus auratus.
• Intramuscular bone induction by the simultaneous administration of recombinant human bone morphogenetic protein 2 and bisphosphonate for autobone graft.
• Intranuclear localization and isoform-dependent translocation of 14-3-3 proteins in human brain with infarction.
• Metastasis to the gluteus maximus muscle from renal cell carcinoma with special emphasis on MRI features.
• Molecular basis of basal cell carcinogenesis in the atomic-bomb survivor population: p53 and PTCH gene alterations.
• Nilvadipine prevents cognitive decline of patients with mild cognitive impairment.
• Photochange in pore diameters of azobenzene-planted mesoporous silica materials.
• Plasma brain natriuretic peptide levels indicating thromboembolism in very elderly patients with non-valvular atrial fibrillation.
• Salicylate-based analgesic balm attenuates pressor responses from skeletal muscle.
• Structures of sugar chains of the subunits of an alpha-amylase inhibitor from Phaseolus vulgaris white kidney beans.
• Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: IV. Isolation and identification of four novel oligosaccharide units derived from the acidic polysaccharide chain.
• Survey of dietary habits in obese patients with type 2 diabetes treated with either OHA or insulin injections in Japan.
• [Diagnosis and treatment using double balloon endoscopy]
• [Efficacy of S-1 combined with weekly Paclitaxel for gastric cancer with cancerous ascites]
• [Lacunar infarcts in the elderly with special reference to their historical aspects]
• [Osteosarcoma and pain]