[Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.]

[Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.] Abstract Text:

Yu-fang Cui,Yan-qing Ding,Ying Zhang,Han Xu,Wei Jin,Xiao-lan Liu,Bo Dong,Jian-ping Mao,Bing-zhi Mao,

OBJECTIVE: To observe the apoptotic characteristics of mouse spleen lymphocyte after lethal dose gamma-irradiation and its relationship to the expression of Bax and Bcl-XL proteins. METHODS: Two hundred and twenty-five second-grade C57 mice were randomly divided into six groups of 0, 6, 9, 12, 15 and 20 Gy. They were sacrificed by dislocation and samples were taken on 1-28 days after whole body single gamma-irradiation. Lymphocyte apoptosis and necrosis were analyzed by TdT-mediated dUTP nick end labeling (TUNEL) and flow cytometry (FCM) techniques. The expression of Bax and Bcl-XL proteins were estimated by immunohistochemical method. RESULTS: (1) The number of peripheral white blood cells of mice increased temporarily at 6 hours after radiation, thereafter, began to decrease rapidly, which reached the minimum on day 7 and recovered normal level basically one month after 6 Gy gamma-irradiation. (2) Apoptotic rate of spleen lymphocytes increased significantly, peaking at 6 hours after radiation, which was found to have a dose-response relationship during 6-24 hours after < or =12 Gy irradiation, but decreased after > or =15 Gy irradiation. (3) It was confirmed by FCM that the apoptotic rate of spleen lymphocytes increased along with the elevation of radiation dose. However, the apoptotic rate began to decrease and the necrotic rate rose distinctively after > or =15 Gy irradiation. The analysis of DNA gel electrophoresis supported above-mentioned results. (4) The expression of Bax protein in spleen lymphocyte enhanced at 6 hours after 6 Gy gamma-irradiation and peaked by 12 Gy-irradiation, showing a dose-dependent pattern, but which was not be found after > or =15 Gy gamma-irradiation. On the other hand, the expression of Bcl-XL protein reduced persistently with the increase of radiation dose, and also presented a better dose-dependent effect after < or =12 Gy irradiation. CONCLUSION: After 6-12 Gy gamma-irradiation, the apoptosis is the major death way of spleen lymphocyte, while both necrosis and apoptosis are important death pathways after > or =15 Gy irradiation. Pro-apoptotic Bax and anti-apoptotic Bcl-XL play an important role in the apoptotic regulation of spleen lymphocytes induced by lethal dose radiation.

[Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.] Publishing Authors By Initials

YF Cui,YQ Ding,Y Zhang,H Xu,W Jin,XL Liu,B Dong,JP Mao,BZ Mao,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese cr

VOLUME: 17

Page Numbers: 109-12

Journal Abbreviation:

ISSN: 1003-0603

DAY: 8

MONTH: Feb

YEAR: 2005

[Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 9887521

[Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.]

AFFILIATION: Department of Immunology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zhongguo Wei Zhong Bing Ji Jiu

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins Related Publications

• Comparative study between coral-mesenchymal stem cells-rhBMP-2 composite and auto-bone-graft in rabbit critical-sized cranial defect model.
• Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffold.
• Effect of cytokines secreted by human adipose stromal cells on endothelial cells.
• Evidence for a distinct group of nestin-immunoreactive neurons within the basal forebrain of adult rats.
• Expression of Delta DNMT3B variants and its association with promoter methylation of p16 and RASSF1A in primary non-small cell lung cancer.
• Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors.
• Matrix and gene expression in the rat cranial base growth plate.
• Phosphorylation of glutamate receptors: a potential mechanism for the regulation of receptor function and psychostimulant action.
• Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells.
• The outcome of orbital decompression for thyroid associated ophthalmopathy.
• Tissue-engineered rabbit cranial suture from autologous fibroblasts and BMP2.
• [A preliminary study of nasoalveolar molding for infants born with cleft lip and palate]
• [Adenoviral-mediated expression of PTEN cDNA induces apoptosis in endometrial carcinoma cells]
• [Analysis of T cell subsets and CD3zeta chain expression in myelodysplastic syndrome patients]
• [Analysis of air pollutants with a combination of GC/FTIR and GC/MS]
• [Apoptotic characteristics of spleen lymphocyte in mice irradiated by lethal dose and its relationship to the expression of Bax and Bcl-XL proteins.]
• [Changes of phosphorylation states of Cx43 protein in CCL-64 cells induced by SiO2]
• [Cloning, expression and purification of human stem cell growth factor cDNA and its species-specificity in hematopoiesis]
• [Effects of infused intravenous ice-cold normal saline on the cerebral perfusion pressure of rabbits with focal cerebral ischemia]
• [Experimental study on osteoinduction of coral composited artificial bone]
• [Follow up study on viruses associated with SARS among the SARS patients]
• [Identification and typing of human enteroviruses using an RT-PCR assay]
• [In vitro cotransfer human multidrug resistance gene (mdr-1) and dihydrofolate reductase gene (DHFR) into human CD(34)(+) progenitor cells to broaden the spectrum of drug resistance]
• [Osteoblasts derived from mesenchymal stem cells harbor immunoregulatory effect]
• [Preliminary study on determination of specific cellular immunity of patients with hepatitis B]
• [Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture]
• [SNP marker and allele-specific diagnostic PCR for authenticating herbs of perilla]
• [Studies on regulation of glutamine synthetase activity from Streptomyces lincolnensis]
• [Study on the relationship between polymorphisms of susceptible genes in coagulation pathway related to pulmonary thromboembolism in Chinese Han population]
• [Therapeutic effects of Trigonella foenum-greacum saponin on rats with alcoholic fatty livers]