Apoptosis is required during early stages of tail regeneration in Xenopus laevis.

Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Research Abstract Details 

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Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Abstract Text:

Ai-Sun Tseng,Dany S Adams,Dayong Qiu,Punita Koustubhan,Michael Levin,

The Xenopus tadpole is able to regenerate its tail, including skin, muscle, notochord, spinal cord and neurons and blood vessels. This process requires rapid tissue growth and morphogenesis. Here we show that a focus of apoptotic cells appears in the regeneration bud within 12 h of amputation. Surprisingly, when caspase-3 activity is specifically inhibited, regeneration is abolished. This is true of tails both before and after the refractory period. Programmed cell death is only required during the first 24 h after amputation, as later inhibition has no effect on regeneration. Inhibition of caspase-dependent apoptosis results in a failure to induce proliferation in the growth zone, a mispatterning of axons in the regenerate, and the appearance of ectopic otoliths in the neural tube, in the context of otherwise normal continued development of the larva. Larvae amputated during the refractory stage exhibit a much broader domain of caspase-3-positive cells, suggesting a window for the amount of apoptosis that is compatible with normal regeneration. These data reveal novel roles for apoptosis in development and indicate that a degree of apoptosis is an early and obligate component of normal tail regeneration, suggesting the possibility of the existence of endogenous inhibitory cells that must be destroyed by programmed cell death for regeneration to occur.

Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Publishing Authors By Initials

AS Tseng,DS Adams,D Qiu,P Koustubhan,M Levin,

For similar animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research abstracts see: animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research

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Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Developmental biology

VOLUME: 301

Page Numbers: 62-9

Journal Abbreviation: Dev. Biol.

ISSN: 0012-1606

DAY: 6

MONTH: 11

YEAR: 2006

Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Information

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LANGUAGE: eng

NlmUniqueID: 372762

Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Keywords Mesh Terms:

KEYWORDS: Xenopus laevis

MESH TERMS: physiology

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Grant and Affiliation Information for Apoptosis is required during early stages of tail regeneration in Xenopus laevis.

AFFILIATION: Center for Regenerative and Developmental Biology, Forsyth Institute, and Developmental Biology Department, Harvard School of Dental Medicine, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NCI

GRANT: CO6RR11244

ACRONYM: CO

MEDLINETA: Dev Biol

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