[Apoptosis-inducing ligand TRAIL can be recruited to lipid rafts]

[Apoptosis-inducing ligand TRAIL can be recruited to lipid rafts] Research Abstract Details 

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[Apoptosis-inducing ligand TRAIL can be recruited to lipid rafts] Abstract Text:

Wei Jia,Ran Zhuang,Qi Li,Yun-Xin Cao,Xue-Song Liu,Chao-Jun Song,Bo-Quan Jin,

AIM: To investigate the relationship between tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) and cell membrane microdomain lipid rafts. METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry. The lipid rafts on K562 cells were detected with FITC-labeled cholera toxin B subunit (CTx-FITC) which bound to the GM1 glycosphingolipid in lipid rafts. The lipid rafts were then cross-linked with an anti-FITC mAb after binding with CTx-FITC. The localization of TRAIL on K562 cells was analyzed with rabbit anti-TRAIL antibody, Cy3-labeled goat-anti-rabbit IgG (Cy3-labeled GAR) and laser scanning confocal microscope. RESULTS: The cross-linking of lipid rafts appeared at 20 minutes, reached peak at 30 minutes, and weakened around 40 minutes. After the cells were labeled with CTx-FITC and anti-FITC mAb TRAIL was aggregated in the cross-linked lipid rafts. CONCLUSION: The anti-FITC mAb could be used for cross-linking of lipid rafts. TRAIL can be recruited to lipid rafts.

[Apoptosis-inducing ligand TRAIL can be recruited to lipid rafts] Publishing Authors By Initials

W Jia,R Zhuang,Q Li,YX Cao,XS Liu,CJ Song,BQ Jin,

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[Apoptosis-inducing ligand TRAIL can be recruited to lipid rafts] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 21

Page Numbers: 1-5

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 4

MONTH: Jan

YEAR: 2005

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LANGUAGE: chi

NlmUniqueID: 101139110

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AFFILIATION: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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