Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin.

Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin. Research Abstract Details 

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Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin. Abstract Text:

Takenori Seki,Tomoya Kunichika,Kiyotaka Watanabe,Koichi Orino,Takenori Seki,Tomoya Kunichika,Kiyotaka Watanabe,Koichi Orino,

Apolipoprotein B (apoB) is known to be a ferritin-binding protein. Here we show that apoB binds to ferritin through hemin-mediated binding. Human apoB bound to bovine spleen, horse spleen, and canine liver ferritins, but did not bind to bovine apoferritin, even after incorporation of iron into it. Incubation of apoferritin with hemin resulted in apoB binding with apoferritin at the same level as with holoferritin. In contrast, hemin inhibited binding of apoB to ferritin. Bovine spleen apoferritin bound biotinylated hemin, and hemin inhibited the binding between the apoferritin and biotinylated hemin, suggesting that ferritin binds hemin directly. ApoB and LDL containing apoB bound biotinylated hemin, and their bindings were also inhibited by hemin, but not protoporphyrin IX. These data demonstrate that binding of apoB to ferritin is mediated through ferritin's binding to hemin, and also that apoB binds hemin directly.

Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin. Publishing Authors By Initials

T Seki,T Kunichika,K Watanabe,K Orino,T Seki,T Kunichika,K Watanabe,K Orino,

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Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biometals : an international journal on the role o

VOLUME: 21

Page Numbers: 61-9

Journal Abbreviation: Biometals

ISSN: 0966-0844

DAY: 14

MONTH: 03

YEAR: 2007

Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin. Information

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LANGUAGE: eng

NlmUniqueID: 9208478

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AFFILIATION: Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori, 034-8628, Japan, orino@vmas.kitasato-u.ac.jp.

Country: Netherlands

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MEDLINETA: Biometals

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