Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma.

Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma. Research Abstract Details 

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Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma. Abstract Text:

Ze Tian,Jianyong Si,Qi Chang,Liang Zhou,Shilin Chen,Peigen Xiao,Erxi Wu,Ze Tian,Jianyong Si,Qi Chang,Liang Zhou,Shilin Chen,Peigen Xiao,Erxi Wu,

BACKGROUND: Medicinal plant is a main source of cancer drug development. Some of the cycloartane triterpenoids isolated from the aerial part of Cimicifuga dahurica showed cytotoxicity in several cancer cell lines. It is of great interest to examine the antiproliferative activity and mechanisms of total triterpenoid glycosides of C. dahurica and therefore might eventually be useful in the prevention or treatment of Hepatoma. METHODS: The total glycosides from the aerial part (TGA) was extracted and its cytotoxicity was evaluated in HepG2 cells and primary cultured normal mouse hepatocytes by an MTT assay. Morphology observation, Annexin V-FITC/PI staining, cell cycle analysis and western blot were used to further elucidate the cytotoxic mechanism of TGA. Implanted mouse H22 hepatoma model was used to demonstrate the tumor growth inhibitory activity of TGA in vivo. RESULTS: The IC50 values of TGA in HepG2 and primary cultured normal mouse hepatocytes were 21 and 105 mug/ml, respectively. TGA induced G0/G1 cell cycle arrest at lower concentration (25 mug/ml), and triggered G2/M arrest and apoptosis at higher concentrations (50 and 100 mug/ml respectively). An increase in the ratio of Bax/Bcl-2 was implicated in TGA-induced apoptosis. In addition, TGA inhibited the growth of the implanted mouse H22 tumor in a dose-dependent manner. CONCLUSION: TGA may potentially find use as a new therapy for the treatment of hepatoma.

Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma. Publishing Authors By Initials

Z Tian,J Si,Q Chang,L Zhou,S Chen,P Xiao,E Wu,Z Tian,J Si,Q Chang,L Zhou,S Chen,P Xiao,E Wu,

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Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC cancer

VOLUME: 7

Page Numbers: 237

Journal Abbreviation: BMC Cancer

ISSN: 1471-2407

DAY: 31

MONTH: 12

YEAR: 2007

Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma. Information

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LANGUAGE: eng

NlmUniqueID: 100967800

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Grant and Affiliation Information for Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma.

AFFILIATION: Children's Hospital Informatics Program at Harvard-MIT Division of Health Sciences and Technology, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA. ze.tian@childrens.harvard.edu

Country: England

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MEDLINETA: BMC Cancer

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